Effect of gallic acid on peptides released by trypsin digestion of bovine α-casein

被引：1

作者：

Lai, Ping ^{[1
]}

Okazawa, Atsushi ^{[1
,2
]}

Izumi, Yoshihiro ^{[1
]}

Bamba, Takeshi ^{[1
]}

Fukusaki, Eiichiro ^{[1
]}

Yoshikawa, Masaaki ^{[3
]}

Kobayashi, Akio ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan

[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Dept Appl Life Sci, Naka Ku, Sakai, Osaka 5998531, Japan

[3] Res Inst Prod Dev, Sakyo Ku, Kyoto 6060805, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2013年 / 115卷 / 03期

关键词：

Angiotensin I-converting enzyme inhibitory activity; alpha-Casein; Gallic acid; Liquid chromatography/ion-trap time-of-flight mass spectrometry (LC/IT-TOF-MS); Methionine sulfoxide; Trypsin digestion; PHENOLIC-COMPOUNDS; CONVERTING-ENZYME; ANTIOXIDANT; PROTEINS; HYDROLYSIS; RESIDUES;

D O I：

10.1016/j.jbiosc.2012.10.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, the effects of gallic acid (GA) on trypsin digestion of commercial alpha-casein (alpha-CN), which contains alpha(s1)-CN and alpha(s2)-CN, and the peptides released during digestion were investigated. Gallic acid showed no effect on the initial rate of digestion. However, the apparent degree of hydrolysis achieved its maximum value after 1 h, then decreased in the presence of GA, suggesting the cross-linking between peptides once released from alpha-CN during digestion. In the presence of GA, three peaks derived from alpha(s1)-CN disappeared and three new peaks appeared in high-performance liquid chromatography (HPLC) analysis. In these peptides, two Met residues corresponding to the Met(135) and Met(196) in alpha(s1)-CN were oxidized to Met sulfoxide residues. The oxidation of Met(196) was quicker than that of Met(135). The inhibitory activity of TTMPLW (alpha(s1)-CN 193-199) against angiotensin I-converting enzyme was reduced slightly by the oxidation of its Met residue. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：259 / 267

页数：9

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