Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

被引：17

作者：

Morimoto, Shota ^{[1
]}

Tomohiro, Takenori ^{[1
]}

Maruyama, Nobuyuki ^{[2
]}

Hatanaka, Yasumaru ^{[1
]}

机构：

[1] Toyama Univ, Grad Sch Med & Pharmaceut Sci, Toyama 9300194, Japan

[2] Kyoto Univ, Grad Sch Agr, Lab Food Qual Design & Dev, Uji, Kyoto 6110011, Japan

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 18期

关键词：

PHOTO-CROSS-LINKING; MASS-SPECTROMETRY; ALPHA'-SUBUNIT; PROTEOMICS; PROBES; DIAZIRINE; REAGENTS; LINKERS; BIOLOGY;

D O I：

10.1039/c3cc38594a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

引用

页码：1811 / 1813

页数：3

共 32 条

[1] Development and application of diazirines in biological and synthetic macromolecular systems [J].

Blencowe, A ;

Hayes, W .

SOFT MATTER, 2005, 1 (03) :178-205

[2] NEW PHOTOLABELING AND CROSS-LINKING METHODS [J].

BRUNNER, J .

ANNUAL REVIEW OF BIOCHEMISTRY, 1993, 62 :483-514

[3] Developing photoactive affinity probes for proteomic profiling: Hydroxamate-based probes for metalloproteases [J].

Chan, EWS ;

Chattopadhaya, S ;

Panicker, RC ;

Huang, X ;

Yao, SQ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (44) :14435-14446

[4] IDENTIFICATION OF THE TARGET OF A TRANSCRIPTION ACTIVATOR PROTEIN BY PROTEIN-PROTEIN PHOTO-CROSS-LINKING [J].

CHEN, Y ;

EBRIGHT, YW ;

EBRIGHT, RH .

SCIENCE, 1994, 265 (5168) :90-92

[5] Aliphatic Diazirines as Photoaffinity Probes for Proteins: Recent Developments [J].

Das, Joydip .

CHEMICAL REVIEWS, 2011, 111 (08) :4405-4417

[6] Review - Mass spectrometry and protein analysis [J].

Domon, B ;

Aebersold, R .

SCIENCE, 2006, 312 (5771) :212-217

[7] Chemical cross-linking with a diazirine photoactivatable cross-linker investigated by MALDI- and ESI-MS/MS [J].

Gomes, Alexandre F. ;

Gozzo, Fabio C. .

JOURNAL OF MASS SPECTROMETRY, 2010, 45 (08) :892-899

[8] Post-biotinylation of photocrosslinking by Staudinger-Bertozzi ligation of preinstalled alkylazide tag [J].

Hashimoto, M ;

Hatanaka, Y .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2005, 53 (11) :1510-1512

[9] Simple synthesis of deuterium and 13C labeled trifluoromethyl phenyldiazirine derivatives as stable isotope tags for mass spectrometry [J].

Hashimoto, M ;

Hatanaka, Y .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2004, 52 (11) :1385-1386

[10]

Hatanaka Y, 1994, Bioorg Med Chem, V2, P1367, DOI 10.1016/S0968-0896(00)82088-X

← 1 2 3 4 →