Proteomic study explores AGR2 as pro-metastatic protein in HCC

被引：22
作者：
Yu, Hongxiu [1 ]
Zhao, Jian [1 ,2 ,3 ]
Lin, Ling [1 ]
Zhang, Yang [1 ]
Zhong, Fan [1 ]
Liu, Yinkun [1 ,4 ]
Yu, Yanyan [1 ]
Shen, Huali [1 ]
Han, Meimei [1 ]
He, Fuchu [1 ,2 ,5 ]
Yang, Pengyuan [1 ,6 ]
机构：
[1] Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China
[2] Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China
[3] Zhejiang Hisun Pharmaceut Corp, Hangzhou, Zhejiang, Peoples R China
[4] Fudan Univ, Zhongshan Hosp, Shanghai 200433, Peoples R China
[5] Beijing Inst Radiat Med, Beijing Prote Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China
[6] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
来源：
MOLECULAR BIOSYSTEMS | 2012年 / 8卷 / 10期
关键词：
HUMAN HEPATOCELLULAR-CARCINOMA; ANTERIOR GRADIENT-2; ESTROGEN-RECEPTOR; GENE-EXPRESSION; HUMAN HOMOLOG; CANCER; IDENTIFICATION; APOPTOSIS; CELLS; ALG-2;
D O I：
10.1039/c2mb25160d
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hepatocellular carcinoma (HCC) is one of the most common and aggressive malignant tumors worldwide. The prognosis of patients with HCC still remains very dismal, mainly due to metastasis. We found that high-expression levels of AGR2 existed in metastatic HCC cell lines and patient samples. Overexpression of AGR2 was found to be correlated to the metastatic status of HCC cells, and inhibition of AGR2 by siRNA resulted in a dramatic decline in invasion abilities in metastatic cells in vitro. Overexpression of AGR2 increased the invasion of HCC cells in vitro and also in vivo with a nude mouse model. The tandem affinity purification (TAP) identified 18 AGR2-binding proteins and IPA analysis revealed that these proteins focus on MAPK and Caspase pathway. Therefore, we speculate that the overexpression of AGR2 can promote HCC metastasis, possibly by affecting MAPK and Caspase pathway through AGR2-interacting proteins.
引用
收藏
页码：2710 / 2718
页数：9
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