Fate and residues of C-14-chloramphenicol in laying chickens

被引:9
作者
Akhtar, MH
ElSooud, KA
Shehata, AM
AnwarulHaq
机构
[1] CAIRO UNIV,FAC VET MED,DEPT PHARMACOL,GIZA,EGYPT
[2] NUCL INST AGR & BIOL,FAISALABAD,PAKISTAN
关键词
chloramphenicol; chickens; metabolism; tissue residues;
D O I
10.1080/03601239609373054
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Studies were conducted to determine the metabolic fate of chloramphenicol (CAP) in White Leghorn using the C-14-labelled compound. In one experiment birds were administered orally via intra-crop, a single dose of 100 mg (equivalent to 66 mg kg(-1) body weight) of CAP containing 14 mu Ci C-14-CAP, and its absorption, elimination and distribution in plasma were recorded. Orally dosed C-14-compound was rapidly absorbed, efficiently distributed in plasma and eliminated in excreta (>70% in 5 hr). After 5 h, CAP equivalent residues in tissues were lower than 15 mu g g(-1) for this treatment. In a second experiment birds were given intra-crop dose of either 0.5 or 5 mg of CAP (each dose contained 2.5 mu Ci C-14-CAP) daily for five consecutive days followed by a seven day withdrawal period and elimination of C-14 in excreta and eggs was monitored. More than 95% of the administered C-14 was eliminated within the first 24 h after dosing. Radiocarbon (C-14) was deposited preferentially in yolks compared to albumen or other tissues. Residues declined when feeding was stopped. Various metabolites were isolated and identified by a combination of TLC, LC, and LC-MS. The main metabolic route of CAP in laying hens appears to be the glucuronidation. Cleavage of the dichloroacetate moiety was only a minor route.
引用
收藏
页码:1061 / 1084
页数:24
相关论文
共 26 条
[1]   GAS-CHROMATOGRAPHIC DETERMINATION OF INCURRED CHLORAMPHENICOL RESIDUES IN EGGS FOLLOWING OPTIMAL EXTRACTION [J].
AKHTAR, MH ;
DANIS, C ;
SAUVE, A ;
BARRY, C .
JOURNAL OF CHROMATOGRAPHY A, 1995, 696 (01) :123-130
[2]  
ALLEN EH, 1985, J ASSOC OFF ANA CHEM, V68, P990
[3]  
AMES TR, 1985, AM J VET RES, V46, P2471
[4]  
ARCHIMBAULT P, 1987, ANN RECH VET, V18, P85
[5]  
BUSCH H, 1967, CHEMOTHERAPY INTRO T, pCH3
[6]  
DELEPINE B, 1993, EURORESIDUE, V2, P267
[7]  
FEDER HM, 1981, REV INFECT DIS, V3, P479
[8]  
Gilman A.G., 1980, PHARM BASIS THERAPEU, V6th
[9]  
GLAZKO AJ, 1950, J BIOL CHEM, V183, P679
[10]  
*JOINT FAO WHO EXP, 1994, JECFA 42 M