Tubular cell HIV-entry through apoptosed CD4 T cells: A novel pathway

被引：17

作者：

Singh, Priyanka ^{[1
]}

Goel, Hersh ^{[1
]}

Husain, Mohammad ^{[1
]}

Lan, Xiqian ^{[1
]}

Mikulak, Joanna ^{[1
]}

Malthotra, Ashwani ^{[1
]}

Teichberg, Saul ^{[1
]}

Schmidtmayerova, Helena ^{[1
]}

Singhal, Pravin C. ^{[1
]}

机构：

[1] Hofstra N Shore Long Isl Jewish Med Sch, Feinstein Inst Med Res, Ctr Excellence Immunol & Inflammat, New Hyde Pk, NY USA

来源：

VIROLOGY | 2012年 / 434卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Human proximal tubular cells; HIV; T cell; Apoptosis; PD-1; receptor; PD-L1; Phagocytosis; Reactive oxygen species; IMMUNODEFICIENCY-VIRUS TYPE-1; HEPATITIS-B-VIRUS; EPITHELIAL-CELLS; EXPRESSION; PD-L1; ANTIGEN; B7-H1; INTERNALIZATION; PHAGOCYTOSIS; RECOGNITION;

D O I：

10.1016/j.virol.2012.09.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We hypothesized that HIV-1 may enter tubular cells by phagocytosis of apoptotic fragments of HIV-1-infected T cells infiltrating tubular interstitium. The study was designed to evaluate the interaction of programmed death-1 (PD-1) receptors on CD4 T cells and programmed death ligand-1 (PD-L1) on tubular cells (HK2 and HRFTEC, primary tubular cells). Co-cultivation of HIV-1 infected lymphocytes (HIV-LY) with HK2s/HRPTECs resulted in T cell apoptosis, uptake of the apoptosed HIV-LY by HK2s/HRPTECs, tubular cell activation and HIV expression. Cytochalasin-B inhibited tubular cell HIV-LY uptake and anti-PD-L1 antibody inhibited HIV-LY apoptosis and tubular cell HIV-LY uptake, activation and HIV expression. These observations do indicate induction of apoptosis of T cells due to interaction of PD-1 and PD-L1 upon co-cultivation and subsequent phygocytosis of HIV-laden apoptotic bodies by tubular cells and thus the transfer of HIV-1 into tubular cells. These findings identify a novel pathway that facilitates HIV-1 entry into tubular cell. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：68 / 77

页数：10

共 47 条

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