Deregulation of dicer and mir-155 expression in liposarcoma

被引：23

作者：

Vincenzi, Bruno ^{[1
]}

Iuliani, Michele ^{[2
]}

Zoccoli, Alice ^{[2
]}

Pantano, Francesco ^{[1
]}

Fioramonti, Marco ^{[2
]}

De Lisi, Delia ^{[1
]}

Frezza, Anna Maria ^{[1
]}

Rabitti, Carla ^{[3
]}

Perrone, Giuseppe ^{[3
]}

Muda, Andrea Onetti ^{[3
]}

Russo, Antonio ^{[5
]}

Giordano, Antonio ^{[6
,7
]}

Santini, Daniele ^{[1
]}

Dei Tos, Angelo Paolo ^{[4
]}

Tonini, Giuseppe ^{[1
]}

机构：

[1] Campus Biomed Univ Rome, Med Oncol, Rome, Italy

[2] Campus Biomed Univ Rome, Translat Oncol Lab, Rome, Italy

[3] Campus Biomed Univ Rome, Pathol, Rome, Italy

[4] Gen Hosp Treviso, Anat Pathol, Treviso, Italy

[5] Univ Palermo, Dept Surg Oncol & Oral Sci, Palermo, Italy

[6] Temple Univ BioLife Sci, Coll Sci & Technol, Sbarro Inst Canc Res & Mol Med, Philadelphia, PA USA

[7] Univ Siena, Dept Med Surg & Neurosci, I-53100 Siena, Italy

来源：

ONCOTARGET | 2015年 / 6卷 / 12期

关键词：

Dicer; Drosha; mir-155; liposarcoma; PRIMARY EXTREMITY LIPOSARCOMA; RESECTION PREDICT PATTERN; CANCER-PATIENTS; DEDIFFERENTIATED LIPOSARCOMA; RETROPERITONEAL LIPOSARCOMA; HISTOLOGIC SUBTYPE; SURVIVAL; DROSHA; MICRORNA; IMPACT;

D O I：

10.18632/oncotarget.3201

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Liposarcoma (LPS) is the most common soft tissue sarcoma. It has been demonstrated that mir-155 was the most overexpressed miRNA in well-differentiated LPS(WDLPS)/dedifferentiated LPS (DDLPS). The aim of this study is to evaluate the involvement of Dicer, Drosha and mir-155 in development of LPS and their possible role in stratification of different histological subtypes. Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors. The experimental data were obtained using qRT-PCR comparing Dicer, Drosha and mir-155 expression levels in tumor samples versus normal fat. The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ. Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS. These findings demonstrate for the first time that Dicer is deregulated in LPS and show that mir-155 is differentially expressed in LPS subgroups and it could be a promising tool to improve LPS disease stratification and differential diagnosis.

引用

页码：10586 / 10591

页数：6

共 26 条

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