Abscisic Acid Mediates Salicylic Acid Induced Chilling Tolerance of Grafted Cucumber by Activating H2O2 Biosynthesis and Accumulation

被引:9
作者
Zhang, Yanyan [1 ,2 ]
Fu, Xin [1 ]
Feng, Yiqing [1 ]
Zhang, Xiaowei [1 ]
Bi, Huangai [1 ]
Ai, Xizhen [1 ]
机构
[1] Shandong Agr Univ, Coll Hort Sci & Engn, Collaborat Innovat Ctr Fruit & Vegetable Qual & Ef, Key Lab Crop Biol & Genet Improvement Hort Crops H, Tai An 271018, Peoples R China
[2] Taian Acad Agr Sci, Tai An 271000, Peoples R China
基金
美国国家科学基金会;
关键词
salicylic acid; abscisic acid; hydrogen peroxide; signal transduction chilling tolerance; cucumber; pumpkin; HYDROGEN-PEROXIDE; PLANT PRODUCTIVITY; LOW-TEMPERATURE; HEAT TOLERANCE; ABA; RESPONSES; STRESS; PHYTOHORMONES; SEEDLINGS; MOLECULE;
D O I
10.3390/ijms232416057
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Grafting is widely applied to enhance the tolerance of some vegetables to biotic and abiotic stress. Salicylic acid (SA) is known to be involved in grafting-induced chilling tolerance in cucumber. Here, we revealed that grafting with pumpkin (Cucurbita moschata, Cm) as a rootstock improved chilling tolerance and increased the accumulation of SA, abscisic acid (ABA) and hydrogen peroxide (H2O2) in grafted cucumber (Cucumis sativus/Cucurbita moschata, Cs/Cm) leaves. Exogenous SA improved the chilling tolerance and increased the accumulation of ABA and H2O2 and the mRNA abundances of CBF1, COR47, NCED, and RBOH1. However, 2-aminoindan-2-phosphonic acid (AIP) and L-a-aminooxy-b-phenylpropionic acid (AOPP) (biosynthesis inhibitors of SA) reduced grafting-induced chilling tolerance, as well as the synthesis of ABA and H2O2, in cucumber leaves. ABA significantly increased endogenous H2O2 production and the resistance to chilling stress, as proven by the lower electrolyte leakage (EL) and chilling injury index (CI). However, application of the ABA biosynthesis inhibitors sodium tungstate (Na2WO4) and fluridone (Flu) abolished grafting or SA-induced H2O2 accumulation and chilling tolerance. SA-induced plant response to chilling stress was also eliminated by N,N '-dimethylthiourea (DMTU, an H2O2 scavenger). In addition, ABA-induced chilling tolerance was attenuated by DMTU and diphenyleneiodonium (DPI, an H2O2 inhibitor) chloride, but AIP and AOPP had little effect on the ABA-induced mitigation of chilling stress. Na2WO4 and Flu diminished grafting- or SA-induced H2O2 biosynthesis, but DMTU and DPI did not affect ABA production induced by SA under chilling stress. These results suggest that SA participated in grafting-induced chilling tolerance by stimulating the biosynthesis of ABA and H2O2. H2O2, as a downstream signaler of ABA, mediates SA-induced chilling tolerance in grafted cucumber plants.
引用
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页数:18
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