Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion

被引：49

作者：

Colombo, Miriam ^{[2
]}

Mazzucchelli, Serena ^{[3
]}

Montenegro, Jose Maria ^{[1
]}

Galbiati, Elisabetta ^{[2
]}

Corsi, Fabio ^{[3
]}

Parak, Wolfgang J. ^{[1
]}

Prosperi, Davide ^{[2
]}

机构：

[1] Univ Marburg, Fachbereich Phys, D-35037 Marburg, Germany

[2] Univ Milano Bicocca, Dipartimento Biotecnol & Biosci, I-20126 Milan, Italy

[3] Univ Milan, Dipartimento Sci Clin Luigi Sacco, I-20157 Milan, Italy

来源：

SMALL | 2012年 / 8卷 / 10期

关键词：

magnetic nanoparticles; magnetofluorescence; fusion proteins; bioconjugation; tumor targeting; MAGNETIC NANOPARTICLES; SELECTIVE IMMOBILIZATION; COVALENT; TECHNOLOGY; EXPRESSION; ANTIBODIES; ACID;

D O I：

10.1002/smll.201102284

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the site-specific covalent conjugation of targeting peptides to nanoparticles. An scFv mutant selective for HER2 tumor antigen is chosen as the targeting ligand. SNAP-scFv is immobilized on magnetofluorescent nanoparticles and its targeting efficiency against HER2-positive cells is assessed by flow cytometry and immunofluorescence. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

收藏

页码：1492 / 1497

页数：6

相关论文

共 29 条

[1] Large-scale bacterial fermentation and isolation of scFv multimers using a heat-inducible bacterial expression vector [J].

Bayly, AM ;

Kortt, AA ;

Hudson, PJ ;

Power, BE .

JOURNAL OF IMMUNOLOGICAL METHODS, 2002, 262 (1-2) :217-227

[2] HER2 Expression in Breast Cancer Cells Is Downregulated Upon Active Targeting by Antibody-Engineered Multifunctional Nanoparticles in Mice [J].

Corsi, Fabio ;

Fiandra, Luisa ;

De Palma, Clara ;

Colombo, Miriam ;

Mazzucchelli, Serena ;

Verderio, Paolo ;

Allevi, Raffaele ;

Tosoni, Antonella ;

Nebuloni, Manuela ;

Clementi, Emilio ;

Prosperi, Davide .

ACS NANO, 2011, 5 (08) :6383-6393

[3]

Haun JB, 2010, NAT NANOTECHNOL, V5, P660, DOI [10.1038/nnano.2010.148, 10.1038/NNANO.2010.148]

[4] Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands [J].

Hodneland, CD ;

Lee, YS ;

Min, DH ;

Mrksich, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5048-5052

[5] SNAP-Tag Technology Mediates Site Specific Conjugation of Antibody Fragments with a Photosensitizer and Improves Target Specific Phototoxicity in Tumor Cells [J].

Hussain, Ahmad Fawzi ;

Kampmeier, Florian ;

von Felbert, Verena ;

Merk, Hans-F. ;

Tur, Mehmet Kemal ;

Barth, Stefan .

BIOCONJUGATE CHEMISTRY, 2011, 22 (12) :2487-2495

[6] A general method for the covalent labeling of fusion proteins with small molecules in vivo [J].

Keppler, A ;

Gendreizig, S ;

Gronemeyer, T ;

Pick, H ;

Vogel, H ;

Johnsson, K .

NATURE BIOTECHNOLOGY, 2003, 21 (01) :86-89

[7] Designed fabrication of multifunctional magnetic gold nanoshells and their application to magnetic resonance imaging and photothermal therapy [J].

Kim, Jaeyun ;

Park, Sungjin ;

Lee, Ji Ean ;

Jin, Seung Min ;

Lee, Jung Hee ;

Lee, In Su ;

Yang, Ilseung ;

Kim, Jun-Sung ;

Kim, Seong Keun ;

Cho, Myung-Haing ;

Hyeon, Taeghwan .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (46) :7754-7758

[8] Covalent and selective immobilization of fusion proteins [J].

Kindermann, M ;

George, N ;

Johnsson, N ;

Johnsson, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (26) :7810-7811

[9] Di-, tri- and tetrameric single chain Fv antibody fragments against human CD19: effect of valency on cell binding [J].

Le Gall, F ;

Kipriyanov, SM ;

Moldenhauer, G ;

Little, M .

FEBS LETTERS, 1999, 453 (1-2) :164-168

[10]

Lee JH, 2007, NAT MED, V13, P95, DOI [10.1038/nm1467, 10.20659/jfp.13.1_95]