High temporal variability in commensal Escherichia coli strain communities of a herbivorous marsupial

被引:21
作者
Blyton, Michaela D. J. [1 ,2 ]
Banks, Sam C. [2 ]
Peakall, Rod [1 ]
Gordon, David M. [1 ]
机构
[1] Australian Natl Univ, Res Sch Biol, Canberra, ACT 0200, Australia
[2] Australian Natl Univ, Fenner Sch Environm & Soc, Canberra, ACT 0200, Australia
基金
澳大利亚研究理事会;
关键词
MOUNTAIN BRUSHTAIL POSSUM; GENETIC-STRUCTURE; TRICHOSURUS-CANINUS; HOUSEHOLD MEMBERS; POPULATION; VIRULENCE; HOST; IDENTIFICATION; COLONIZATION; PERSISTENCE;
D O I
10.1111/1462-2920.12088
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Although Escherichia coli is an important model organism for bacterial research, few studies have explored the nature of temporal variation in E. coli strains within the intestinal tracts of host individuals. In this study the E. coli strains of 54 mountain brushtail possums were sampled on four occasions during a year. This allowed temporal changes to be quantified both at the host population level and within individuals. Escherichia coli strains were identified using a combination of rep-PCR profiles from two primers (CGG and ERIC) and phylogenetic group assigned by quadruplex PCR. The study revealed considerable changes in community structure within individuals among all time periods. In fact, temporal variation within individuals accounted for more of the variation in E. coli community structure than differences between animals. In contrast to the within-host dynamics, there were no significant differences among the time periods at the host population level. It was also found that there was no effect of host age or sex on strain community structure within host individuals. These findings highlight the importance of temporal variation in the ecology of E. coli, while the methods applied in this study may serve as a foundation for further work in this area.
引用
收藏
页码:2162 / 2172
页数:11
相关论文
共 38 条
  • [1] (CGG)4-Based PCR as a Novel Tool for Discrimination of Uropathogenic Escherichia coli Strains: Comparison with Enterobacterial Repetitive Intergenic Consensus-PCR
    Adamus-Bialek, Wioletta
    Wojtasik, Arkadiusz
    Majchrzak, Marta
    Sosnowski, Marek
    Parniewski, Pawel
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (12) : 3937 - 3944
  • [2] Persistence and differential survival of fecal indicator bacteria in subtropical waters and sediments
    Anderson, ML
    Whitlock, JE
    Harwood, VJ
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (06) : 3041 - 3048
  • [3] COMPETITION OF 2 MICROBIAL-POPULATIONS FOR A SINGLE RESOURCE IN A CHEMOSTAT WHEN ONE OF THEM EXHIBITS WALL ATTACHMENT
    BALTZIS, BC
    FREDRICKSON, AG
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (10) : 2419 - 2439
  • [4] Moving pictures of the human microbiome
    Caporaso, J. Gregory
    Lauber, Christian L.
    Costello, Elizabeth K.
    Berg-Lyons, Donna
    Gonzalez, Antonio
    Stombaugh, Jesse
    Knights, Dan
    Gajer, Pawel
    Ravel, Jacques
    Fierer, Noah
    Gordon, Jeffrey I.
    Knight, Rob
    [J]. GENOME BIOLOGY, 2011, 12 (05):
  • [5] CAUGANT DA, 1981, GENETICS, V98, P467
  • [6] The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups
    Clermont, Olivier
    Christenson, Julia K.
    Denamur, Erick
    Gordon, David M.
    [J]. ENVIRONMENTAL MICROBIOLOGY REPORTS, 2013, 5 (01): : 58 - 65
  • [7] Characterization of the cryptic Escherichia lineages: rapid identification and prevalence
    Clermont, Olivier
    Gordon, David M.
    Brisse, Sylvain
    Walk, Seth T.
    Denamur, Erick
    [J]. ENVIRONMENTAL MICROBIOLOGY, 2011, 13 (09) : 2468 - 2477
  • [8] Morphology and function of the gastrointestinal tract of Australian folivorous possums
    Crowe, O
    Hume, ID
    [J]. AUSTRALIAN JOURNAL OF ZOOLOGY, 1997, 45 (04) : 357 - 368
  • [9] Assigning Escherichia coli strains to phylogenetic groups:: multi-locus sequence typing versus the PCR triplex method
    Gordon, David M.
    Clermont, Olivier
    Tolley, Heather
    Denamur, Erick
    [J]. ENVIRONMENTAL MICROBIOLOGY, 2008, 10 (10) : 2484 - 2496
  • [10] Influence of the age and sex of human hosts on the distribution of Escherichia coli ECOR groups and virulence traits
    Gordon, DM
    Stern, SE
    Collignon, PJ
    [J]. MICROBIOLOGY-SGM, 2005, 151 : 15 - 23