Methotrexate influx via folate transporters into alveolar epithelial cell line A549

被引：15

作者：

Kawami, Masashi ^{[1
]}

Miyamoto, Mioka ^{[1
]}

Yumoto, Ryoko ^{[1
]}

Takano, Mikihisa ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Pharmaceut & Therapeut, Minami Ku, Hiroshima 7348553, Japan

来源：

DRUG METABOLISM AND PHARMACOKINETICS | 2015年 / 30卷 / 04期

基金：

日本学术振兴会;

关键词：

Alveolar epithelial cells; Folic acid; Methotrexate; Proton-coupled folate transporter; Reduced folate carrier; CARRIER-MEDIATED TRANSPORT; RHEUMATOID-ARTHRITIS; INTESTINAL-ABSORPTION; MOLECULAR-BASIS; PH-DEPENDENCE; FOLIC-ACID; IDENTIFICATION; RECEPTOR; PHARMACOKINETICS; SULFASALAZINE;

D O I：

10.1016/j.dmpk.2015.04.005

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Methotrexate (MTX), a drug used for the treatment of certain cancers as well as rheumatoid arthritis, sometimes induces serious interstitial lung injury. Although lung toxicity of MTX is related to its accumulation, the information concerning MTX transport in the lungs is lacking. In this study, we investigated the mechanisms underlying MTX influx into human alveolar epithelial cell line A549. MTX influx into A549 cells was time-, pH-, and temperature-dependent and showed saturation kinetics. The influx was inhibited by folic acid with IC50 values of 256.1 mu M at pH 7.4 and 1.6 mu M at pH 5.5, indicating that the mechanisms underlying MTX influx would be different at these pHs. We then examined the role of two folate transporters in MTX influx, reduced folate carrier (RFC) and proton-coupled folate transporter (PCFT). The expression of RFC and PCFT mRNAs in A549 cells was confirmed by reverse transcription polymerase chain reaction. In addition, MTX influx was inhibited by thiamine monophosphate, an RFC inhibitor, at pH 7.4, and by sulfasalazine, a PCFT inhibitor, at pH 5.5. These results indicated that RFC and PCFT are predominantly involved in MTX influx into A549 cells at pH 7.4 and pH 5.5, respectively. Copyright (C) 2015, The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.

引用

页码：276 / 281

页数：6

共 39 条

[31] Characterization of a folate transporter in HeLa cells with a low pH optimum and high affinity for pemetrexed distinct from the reduced folate carrier [J].