Salicylic acid improves the salinity tolerance of Medicago sativa in symbiosis with Sinorhizobium meliloti by preventing nitrogen fixation inhibition

被引:84
作者
Palma, F. [1 ]
Lopez-Gomez, M. [1 ]
Tejera, N. A. [1 ]
Lluch, C. [1 ]
机构
[1] Univ Granada, Fac Ciencias, Dept Fisiol Vegetal, E-18071 Granada, Spain
关键词
Salicylic acid; Salt stress; Medicago sativa; Polyamines; Nodule; BEAN PHASEOLUS-VULGARIS; SALT STRESS; EXOGENOUS APPLICATION; POLYAMINE METABOLISM; DEFENSE RESPONSES; CATALASE ACTIVITY; ACTIVE OXYGEN; HIGHER-PLANTS; ETHYLENE; PEA;
D O I
10.1016/j.plantsci.2013.03.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this work we have investigated the contribution of pretreatment with 0.1 and 0.5 mM salicylic acid (SA) to the protection against salt stress in root nodules of Medicago sativa in symbiosis with Sinorhizobium meliloti. SA alleviated the inhibition induced by salinity in the plant growth and photosynthetic capacity of M. sativa-S. meliloti symbiosis. In addition, SA prevented the inhibition of the nitrogen fixation capacity under salt stress since nodule biomass was not affected by salinity in SA pretreated plants. Antioxidant enzymes peroxidase (PDX), superoxide dismutase (SOD), ascorbate peroxidase (APX), dehidroascorbate reductase (DHAR) and glutathione reductase (GR), key in the main pathway that scavenges H2O2 in plants, were induced by SA pretreatments which suggest that SA may participate in the redox balance in root nodules under salt stress. Catalase activity (CAT) was inhibited around 40% by SA which could be behind the increase of H2O2 detected in nodules of plants pretreated with SA. The accumulation of polyamines (PAs) synthesized in response to salinity was prevented by SA which together with the induction of 1-aminocyclopropane-l-carboxylic acid (ACC) content suggest the prevalence of the ethylene signaling pathway induced by SA in detriment of the synthesis of PAs. In conclusion, SA alleviated the negative effect of salt stress in the M. sativa-S. meliloti symbiosis through the increased level of nodule biomass and the induction of the nodular antioxidant metabolism under salt stress. The H2O2 accumulation and the PAs inhibition induced by SA in nodules of M. sativa suggest that SA activates a hypersensitive response dependent on ethylene. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:75 / 82
页数:8
相关论文
共 57 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2]   The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat [J].
Alexieva, V ;
Sergiev, I ;
Mapelli, S ;
Karanov, E .
PLANT CELL AND ENVIRONMENT, 2001, 24 (12) :1337-1344
[3]   Exogenous treatment with Salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to Paraquat [J].
Ananieva, EA ;
Christov, KN ;
Popova, LP .
JOURNAL OF PLANT PHYSIOLOGY, 2004, 161 (03) :319-328
[4]   Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress? [J].
Arfan, Muhammad ;
Athar, Habib R. ;
Ashraf, Muhammad .
JOURNAL OF PLANT PHYSIOLOGY, 2007, 164 (06) :685-694
[5]  
Axelrod B., 1981, METHODS ENZYMOLOGY, V71, P441, DOI [10.1016/0076-6879(81)71055-3, DOI 10.1016/0076-6879(81)71055-3]
[6]   Salicylic acid negatively affects the response to salt stress in pea plants [J].
Barba-Espin, G. ;
Clemente-Moreno, M. J. ;
Alvarez, S. ;
Garcia-Legaz, M. F. ;
Hernandez, J. A. ;
Diaz-Vivancos, P. .
PLANT BIOLOGY, 2011, 13 (06) :909-917
[7]   ASSAYING FOR SUPEROXIDE-DISMUTASE ACTIVITY - SOME LARGE CONSEQUENCES OF MINOR CHANGES IN CONDITIONS [J].
BEYER, WF ;
FRIDOVICH, I .
ANALYTICAL BIOCHEMISTRY, 1987, 161 (02) :559-566
[8]   Ethylene: A gaseous signal molecule in plants [J].
Bleecker, AB ;
Kende, H .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2000, 16 :1-+
[9]   Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings [J].
Borsani, O ;
Valpuesta, V ;
Botella, MA .
PLANT PHYSIOLOGY, 2001, 126 (03) :1024-1030
[10]   Polyamines and environmental challenges: recent development [J].
Bouchereau, A ;
Aziz, A ;
Larher, F ;
Martin-Tanguy, J .
PLANT SCIENCE, 1999, 140 (02) :103-125