Design of biostable scaffold based on collagen crosslinked by dialdehyde chitosan with presence of gallic acid

In this study, we have prepared the biostable collagen scaffold which is crosslinked by dialdehyde chitosan (DAC) with presence of Gallic acid (GA) and characterized its physico-chemical, biostable and biocompatible properties. The digital photographic and scanning electron microscopic (SEM) images of the prepared collagen scaffold is exposed well with properly oriented interconnected porous natured structure. The appearance of diffraction peaks showed slightly crystalline characteristic when compared to others. The differential scanning calorimetric (DSC) and thermogravimetric analysis (TGA) measurements indicates well significantly increased denaturation temperature (TD) and decreased decomposition rate. FT-IR result suggests the structural integrity of collagen which favours the molecular stability. The dialdehyde groups from DAC crosslinked with collagen functional groups that increase the molecular crosslinking owing to the large number of amino groups in its molecular chain. This scaffold exhibited 87% resistance against collagenolytic degradation by collagenase. The results showed that the improved biostability which prevents the free access of the collagenase to binds with the collagen triple helical chains. This scaffold confirm high biocompatibilities; enhanced cell proliferation and adhesions properties. This results gains new insight into the collagen scaffold to improves the biostability. This could be suitable method to preparation of collagenous biomaterials for tissue engineering applications. (C) 2019 Elsevier B.V. All rights reserved.