Impact of Thermal Degradation of Cyanidin-3-O-Glucoside of Haskap Berry on Cytotoxicity of Hepatocellular Carcinoma HepG2 and Breast Cancer MDA-MB-231 Cells

被引:32
作者
Pace, Eric [1 ]
Jiang, Yuanyuan [1 ,2 ]
Clemens, Amy [1 ]
Crossman, Tennille [1 ]
Rupasinghe, H. P. Vasantha [1 ]
机构
[1] Dalhousie Univ, Fac Agr, Dept Plant Food & Environm Sci, Truro, NS B2N 5E3, Canada
[2] Sichuan Agr Univ, Coll Sci, Yaan 625014, Peoples R China
基金
加拿大自然科学与工程研究理事会;
关键词
anthocyanin; food processing; antioxidant; cancer; cell viability; phenolic compounds; LONICERA-CAERULEA L; IN-VITRO; PROTOCATECHUIC ACID; ANTIOXIDANT CAPACITY; OXIDATIVE STRESS; ANTHOCYANINS; VIVO; POLYPHENOLS; STABILITY; FRUIT;
D O I
10.3390/antiox7020024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cyanidin-3-O-glucoside (C3G), the predominant anthocyanin in haskap berries (Lonicera caerulea L.), possesses antioxidant and many other biological activities. This study investigated the impact of temperature and pH on the degradation of the C3G-rich haskap fraction. The effect of the thermal degradation products on the viability of hepatocellular carcinoma HepG2 and breast cancer MDA-MB-231 cells was also studied in vitro. Using column chromatography, the C3G-rich fraction was isolated from acetone extracts of haskap berries. The C3G stability in these fractions was studied under elevated temperatures (70 degrees C and 90 degrees C) at three different pH values (2.5, 4, and 7) by monitoring the concentration of C3G and its major degradation products, protocatechuic acid (PCA) and phloroglucinaldehyde (PGA), using liquid chromatography mass spectrometry. Significant degradation of C3G was observed at elevated temperatures and at neutral pH. Conversely, the PCA and PGA concentration increased at higher pH and temperature. Similar to C3G, neutral pH also has a prominent effect on the degradation of PGA, which is further accelerated by heating. The C3G-rich fraction exhibited dose-dependent inhibitory effects on cell metabolic activity when the HepG2 cells were exposed for 48 h. Interestingly, PGA but not PCA exhibited cytotoxic effects against both MDA-MB-231 and HepG2 cells. The results suggest that thermal food processing of haskap could influence its biological properties due to the degradation of C3G.
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页数:13
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共 39 条
[1]   Chemopreventive role of anthocyanins in atherosclerosis via activation of Nrf2-ARE as an indicator and modulator of redox [J].
Aboonabi, Anahita ;
Singh, Indu .
BIOMEDICINE & PHARMACOTHERAPY, 2015, 72 :30-36
[2]  
Arslan D., 2015, Agronomy Research, V13, P892
[3]   In vitro cytotoxicity of protocatechuic acid to city cultured human cells from oral tissue:: Involvement in oxidative stress [J].
Babich, H ;
Sedletcaia, A ;
Kenigsberg, B .
PHARMACOLOGY & TOXICOLOGY, 2002, 91 (05) :245-253
[4]   Antioxidant and cytoprotective properties of partridgeberry polyphenols [J].
Bhullar, Khushwant S. ;
Rupasinghe, H. P. Vasantha .
FOOD CHEMISTRY, 2015, 168 :595-605
[5]   Stability of copigmented anthocyanins and ascorbic acid in a grape juice model system [J].
Brenes, CH ;
del Pozo-Insfran, D ;
Talcott, ST .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (01) :49-56
[6]   Haskap Berries (Lonicera caerulea L.)-a Critical Review of Antioxidant Capacity and Health-Related Studies for Potential Value-Added Products [J].
Celli, Giovana Bonat ;
Ghanem, Amyl ;
Brooks, Marianne Su Ling .
FOOD AND BIOPROCESS TECHNOLOGY, 2014, 7 (06) :1541-1554
[7]   Anticancer Activities of an Anthocyanin-Rich Extract From Black Rice Against Breast Cancer Cells In Vitro and In Vivo [J].
Chang Hui ;
Yu Bin ;
Yu Xiaoping ;
Yi Long ;
Chen Chunye ;
Mi Mantian ;
Ling Wenhua .
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2010, 62 (08) :1128-1136
[8]   Characterization and quantification of Anthocyanins and polyphenolics in blue honeysuckle (Lonicera caerulea L.) [J].
Chaovanalikit, A ;
Thompson, MM ;
Wrolstad, RE .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (04) :848-852
[9]   Phytochemical properties and antioxidant capacities of various colored berries [J].
Chen, Liang ;
Xin, Xiulan ;
Yuan, Qipeng ;
Su, Donghai ;
Liu, Wei .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2014, 94 (02) :180-188
[10]   Human metabolism and elimination of the anthocyanin, cyanidin-3-glucoside: a 13C-tracer study [J].
Czank, Charles ;
Cassidy, Aedin ;
Zhang, Qingzhi ;
Morrison, Douglas J. ;
Preston, Tom ;
Kroon, Paul A. ;
Botting, Nigel P. ;
Kay, Colin D. .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 2013, 97 (05) :995-1003