Secretion of Insulinotropic Proteins by Commensal Bacteria: Rewiring the Gut To Treat Diabetes

被引:59
作者
Duan, Faping [1 ]
Curtis, Katherine L. [1 ]
March, John C. [1 ]
机构
[1] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA
关键词
D O I
10.1128/AEM.01019-08
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Here, we show that commensal bacteria can stimulate intestinal epithelial cells to secrete insulin in response to glucose. Commensal strains were engineered to secrete the insulinotropic proteins GLP-1 and PDX-1. Epithelia stimulated by engineered strains and glucose secreted up to 1 ng ml(-1) of insulin with no significant background secretion.
引用
收藏
页码:7437 / 7438
页数:2
相关论文
共 15 条
  • [1] Genetically modified probiotics in foods
    Ahmed, FE
    [J]. TRENDS IN BIOTECHNOLOGY, 2003, 21 (11) : 491 - 497
  • [2] Biology of incretins: GLP-1 and GIP
    Baggio, Laurie L.
    Drucker, Daniel J.
    [J]. GASTROENTEROLOGY, 2007, 132 (06) : 2131 - 2157
  • [3] Effects of age and sex on postprandial glucose metabolism - Differences in glucose turnover, insulin secretion, insulin action, and hepatic insulin extraction
    Basu, Rita
    Man, Chiara Dalla
    Campioni, Marco
    Basu, Ananda
    Klee, George
    Toffolo, Gianna
    Cobelli, Claudio
    Rizza, Robert A.
    [J]. DIABETES, 2006, 55 (07) : 2001 - 2014
  • [4] Modulation of allergic immune responses by mucosal application of recombinant lactic acid bacteria producing the major birch pollen allergen Bet v 1
    Daniel, C.
    Repa, A.
    Wild, C.
    Pollak, A.
    Pot, B.
    Breiteneder, H.
    Wiedermann, U.
    Mercenier, A.
    [J]. ALLERGY, 2006, 61 (07) : 812 - 819
  • [5] Interrupting Vibrio cholerae infection of human epithelial cells with engineered commensal bacterial signaling
    Duan, Faping
    March, John C.
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 2008, 101 (01) : 128 - 134
  • [6] Engineering of the gut commensal bacterium Bacteroides ovatus to produce and secrete biologically active murine interleukin-2 in response to xylan
    Farrar, MD
    Whitehead, TR
    Lan, J
    Dilger, P
    Thorpe, R
    Holland, KT
    Carding, SR
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2005, 98 (05) : 1191 - 1197
  • [7] Glucagon-like peptide-1-(7-36)amide is transformed to glucagon-like peptide-1-(9-36)amide by dipeptidyl peptidase IV in the capillaries supplying the L cells of the porcine intestine
    Hansen, L
    Deacon, CF
    Orskov, C
    Holst, JJ
    [J]. ENDOCRINOLOGY, 1999, 140 (11) : 5356 - 5363
  • [8] Hartmann M, 2004, INT J MED MICROBIOL, V294, P198
  • [9] Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei
    Hazebrouck, Stephane
    Pothelune, Laetitia
    Azevedo, Vasco
    Corthier, Gerard
    Wal, Jean-Michel
    Langella, Philippe
    [J]. MICROBIAL CELL FACTORIES, 2007, 6 (1)
  • [10] Pancreatic epithelial cells can be converted into insulin-producing cells by GLP-1 in conjunction with virus-mediated gene transfer of pdx-1
    Koizumi, M
    Doi, R
    Fujimoto, K
    Ito, D
    Toyoda, E
    Mori, T
    Kami, K
    Kawaguchi, Y
    Gittes, GK
    Imamura, M
    [J]. SURGERY, 2005, 138 (02) : 125 - 133