Secretion of Insulinotropic Proteins by Commensal Bacteria: Rewiring the Gut To Treat Diabetes

被引：61

作者：

Duan, Faping ^{[1
]}

Curtis, Katherine L. ^{[1
]}

March, John C. ^{[1
]}

机构：

[1] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 23期

关键词：

D O I：

10.1128/AEM.01019-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here, we show that commensal bacteria can stimulate intestinal epithelial cells to secrete insulin in response to glucose. Commensal strains were engineered to secrete the insulinotropic proteins GLP-1 and PDX-1. Epithelia stimulated by engineered strains and glucose secreted up to 1 ng ml(-1) of insulin with no significant background secretion.

引用

页码：7437 / 7438

页数：2

共 15 条

[1] Genetically modified probiotics in foods [J].

Ahmed, FE .

TRENDS IN BIOTECHNOLOGY, 2003, 21 (11) :491-497

[2] Biology of incretins: GLP-1 and GIP [J].

Baggio, Laurie L. ;

Drucker, Daniel J. .

GASTROENTEROLOGY, 2007, 132 (06) :2131-2157

[3] Effects of age and sex on postprandial glucose metabolism - Differences in glucose turnover, insulin secretion, insulin action, and hepatic insulin extraction [J].

Basu, Rita ;

Man, Chiara Dalla ;

Campioni, Marco ;

Basu, Ananda ;

Klee, George ;

Toffolo, Gianna ;

Cobelli, Claudio ;

Rizza, Robert A. .

DIABETES, 2006, 55 (07) :2001-2014

[4] Modulation of allergic immune responses by mucosal application of recombinant lactic acid bacteria producing the major birch pollen allergen Bet v 1 [J].

Daniel, C. ;

Repa, A. ;

Wild, C. ;

Pollak, A. ;

Pot, B. ;

Breiteneder, H. ;

Wiedermann, U. ;

Mercenier, A. .

ALLERGY, 2006, 61 (07) :812-819

[5] Interrupting Vibrio cholerae infection of human epithelial cells with engineered commensal bacterial signaling [J].

Duan, Faping ;

March, John C. .

BIOTECHNOLOGY AND BIOENGINEERING, 2008, 101 (01) :128-134

[6] Engineering of the gut commensal bacterium Bacteroides ovatus to produce and secrete biologically active murine interleukin-2 in response to xylan [J].

Farrar, MD ;

Whitehead, TR ;

Lan, J ;

Dilger, P ;

Thorpe, R ;

Holland, KT ;

Carding, SR .

JOURNAL OF APPLIED MICROBIOLOGY, 2005, 98 (05) :1191-1197

[7] Glucagon-like peptide-1-(7-36)amide is transformed to glucagon-like peptide-1-(9-36)amide by dipeptidyl peptidase IV in the capillaries supplying the L cells of the porcine intestine [J].

Hansen, L ;

Deacon, CF ;

Orskov, C ;

Holst, JJ .

ENDOCRINOLOGY, 1999, 140 (11) :5356-5363

[8]

Hartmann M, 2004, INT J MED MICROBIOL, V294, P198

[9] Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei [J].

Hazebrouck, Stephane ;

Pothelune, Laetitia ;

Azevedo, Vasco ;

Corthier, Gerard ;

Wal, Jean-Michel ;

Langella, Philippe .

MICROBIAL CELL FACTORIES, 2007, 6 (1)

[10] Pancreatic epithelial cells can be converted into insulin-producing cells by GLP-1 in conjunction with virus-mediated gene transfer of pdx-1 [J].

Koizumi, M ;

Doi, R ;

Fujimoto, K ;

Ito, D ;

Toyoda, E ;

Mori, T ;

Kami, K ;

Kawaguchi, Y ;

Gittes, GK ;

Imamura, M .

SURGERY, 2005, 138 (02) :125-133

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