Mapping of the antigenic regions of streptokinase in humans after streptokinase therapy

被引：16
作者：
Torréns, I
Reyes, O
Ojalvo, AG
Seralena, A
Chinea, G
Cruz, LJ
de la Fuente, J
机构：
[1] Ctr Ingn Genet & Biotecnol, Div Pharmaceut, Havana, Cuba
[2] Ctr Ingn Genet & Biotecnol, Div Phys Chem, Havana, Cuba
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 259卷 / 01期
关键词：
D O I：
10.1006/bbrc.1999.0747
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Streptokinase (SK) is efficaciously used as a thrombolytic drug for the treatment of myocardial infarction. Being a bacterial protein, SK is immunogenic in humans. Therefore, resulting from SK therapy, patients become immunized and anti-SK antibody (Ab) titers rise post-treatment. High Ab titers might provoke severe immune reactions during SK therapy and neutralize SK activity, preventing effective thrombolysis. Spot synthesis combined with peptide library techniques is a useful tool for studying protein-peptide interactions on continuous cellulose membranes. Here, we report on the mapping of antigenic regions of SK using a spot-synthesized peptide library and human total sera from patients receiving SK therapy. All tested samples have high anti-SK Ab titers and most of them show significant SK neutralizing capacity. Individual variations in peptide recognition were detected. However, patients treated with SK tend, in general, to show a common regional binding pattern, including residues 1-20, 130-149, 170-189, and 390-399. This is the first study reporting the probing of a cellulose-bound set of peptides with total human sera. (C) 1999 Academic Press.
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收藏
页码：162 / 168
页数：7
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