In ovo injection of ascorbic acid modulates antioxidant defense system and immune gene expression in newly hatched local Chinese yellow broiler chicks

被引：31
作者：
El-Senousey, H. K. [1 ,2 ,3 ]
Chen, B. [1 ,2 ]
Wang, J. Y. [1 ,2 ]
Atta, A. M. [3 ]
Mohamed, F. R. [3 ]
Nie, Q. H. [1 ,2 ]
机构：
[1] South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
[2] South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China
[3] Cairo Univ, Fac Agr, Dept Anim Prod, Giza 12613, Egypt
来源：
POULTRY SCIENCE | 2018年 / 97卷 / 02期
关键词：
antioxidant; ascorbic acid; broiler chick; in ovo injection; immune- related gene; VITAMIN-C; MEAT QUALITY; LIPOIC ACID; PERFORMANCE; HEAT-SHOCK-PROTEIN-70; HATCHABILITY; CYTOKINES; EMBRYO;
D O I：
10.3382/ps/pex310
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The present study was conducted to investigate the effects of in ovo injection of ascorbic acid on antioxidant capacity and immune-related gene expression in the newly hatched local Chinese yellow broiler chicks. Fertile Chinese yellow broiler eggs (n = 90) were assigned to three equal groups. The first group was a non-injected control group. The second group was another control group where the eggs were injected with saline in the air sac after 18 days of incubation. The third group was injected with 3 mg/egg AA in the air sac after 18 days of incubation. In ovo injection of 3 mg/egg AA significantly (P < 0.0001) increased plasma glutathione peroxidase (GSH-PX), total an-tioxidant capacity (T-AOC), and significantly reduced plasma malondialdehyde (MDA) at 1 d old. Moreover, in ovo injection of 3 mg/egg AA significantly increased mRNA expression of glutathione peroxidase (GSH-PX) and superoxide Dismutase (SOD) in the chick's spleen. Additionally, the mRNA level of interleukin 1 beta (IL1 beta), interleukin 6 (IL-6), and tumour necrosis factor alpha (TNF-alpha) in the spleen were significantly decreased (P < 0.0001), which indicates an improvement in chick's immunity. In conclusion, our data suggest that in ovo injection of AA at 3 mg/egg enhance antioxidant defense system and immune system for newly hatched chicks.
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收藏
页码：425 / 429
页数：5
相关论文
共 30 条
[11]   Facultative and obligatory thermogenesis in young birds: a cautionary note [J].
Hohtola, E .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2002, 131 (04) :733-739
[12]   Molecular cloning and characterization of chicken lipopolysaccharide-induced TNF-α factor (LITAF) [J].
Hong, Yeong Ho ;
Lillehoj, Hyun S. ;
Lee, Sung Hyen ;
Park, Dong Woon ;
Lillehoj, Erik P. .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2006, 30 (10) :919-929
[13]   Effects of ascorbic acid and -lipoic acid on performance and meat quality of broilers subjected to heat stress [J].
Imik, H. ;
Ozlu, H. ;
Gumus, R. ;
Atasever, M. Aydemir ;
Urcar, S. ;
Atasever, M. .
BRITISH POULTRY SCIENCE, 2012, 53 (06) :800-808
[14]   Effects of Vitamin C or E on the Pro-inflammatory Cytokines, Heat Shock Protein 70 and Antioxidant Status in Broiler Chicks under Summer Conditions [J].
Jang, In-Surk ;
Ko, Young-Hyun ;
Moon, Yang-Soo ;
Sohn, Sea-Hwan .
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 2014, 27 (05) :749-756
[15]   Prospects of in ovo feeding and nutrient supplementation for poultry: the science and commercial applications - a review [J].
Kadam, Mukund M. ;
Barekatain, Mohammad R. ;
Bhanja, Subrat K. ;
Iji, Paul A. .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2013, 93 (15) :3654-3661
[16]   Prospects for understanding immune-endocrine interactions in the chicken [J].
Kaiser, Pete ;
Wu, Zhiguang ;
Rothwell, Lisa ;
Fife, Mark ;
Gibson, Mark ;
Poh, Tuang-Yeow ;
Shini, Agim ;
Bryden, Wayne ;
Shini, Shaniko .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2009, 163 (1-2) :83-91
[17]   Antioxidant down-regulates interleukin-18 expression in asthma [J].
Lee, Kyung Sun ;
Kim, So Ri ;
Park, Seoung Ju ;
Min, Kyung Hoon ;
Lee, Ka Young ;
Jin, Sun Mi ;
Yoo, Wan Hee ;
Lee, Yong Chul .
MOLECULAR PHARMACOLOGY, 2006, 70 (04) :1184-1193
[18]   Effects of in ovo injection with selenium on immune and antioxidant responses during experimental necrotic enteritis in broiler chickens [J].
Lee, S. H. ;
Lillehoj, H. S. ;
Jang, S. I. ;
Jeong, M. S. ;
Xu, S. Z. ;
Kim, J. B. ;
Park, H. J. ;
Kim, H. R. ;
Lillehoj, E. P. ;
Bravo, D. M. .
POULTRY SCIENCE, 2014, 93 (05) :1113-1121
[19]   Effects of Dietary Manganese on Cu, Fe, Zn, Ca, Se, IL-1β, and IL-2 Changes of Immune Organs in Cocks [J].
Liu, Xiaofei ;
Li, Zhipeng ;
Han, Chunran ;
Zhang, Ziwei ;
Xu, Shiwen .
BIOLOGICAL TRACE ELEMENT RESEARCH, 2012, 148 (03) :336-344
[20]   Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].
Livak, KJ ;
Schmittgen, TD .
METHODS, 2001, 25 (04) :402-408
123