Development of low matrix effects method for the analysis of bisphenol A and bisphenol S in aquatic products by immunoaffinity purification

被引：6

作者：

Shan, Wenchong ^{[1
]}

Yao, Kai ^{[1
]}

Wen, Kai ^{[1
]}

Shao, Bing ^{[1
,2
]}

机构：

[1] China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Coll Vet Med, Beijing 100193, Peoples R China

[2] Beijing Ctr Dis Prevent & Control, Beijing Key Lab Diagnost & Traceabil Technol Food, Beijing 100013, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2019年 / 1109卷

关键词：

Bisphenol A; Bisphenol S; Immunoaffinity column; Ultra-high performance liquid; chromatography-tandem mass spectrometry; Aquatic product; SOLID-PHASE EXTRACTION; SELECTIVE EXTRACTION; PAPER PRODUCTS; CHROMATOGRAPHY; ANALOGS; WATER; BPA; FOODSTUFFS; HPLC; SPECTROMETRY;

D O I：

10.1016/j.jchromb.2019.01.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a high sensitive and reproducible method based on immunoaffinity cleanup followed by ultra performance liquid chromatography-tandem mass spectrometry has been developed for the simultaneous determination bisphenol A (SPA) and bisphenol S (BPS) in aquatic products. The immunoaffinity column was prepared by coupling N-Hydroxysuccinimide-activated Sepharose 4B gel with monoclonal antibodies of BPA and BPS, which can selectively recognize the target analytes to achieve a low matrix interference. The matrix effects of different aquatic products were all less than +/- 10%. The limits of detection in four kinds of aquatic products was 0.060 mu g/kg for SPA and 0.012 mu g/kg for BPS. The recoveries of SPA and BPS in matrices varied from 74% to 106% with relative standard deviations < 12%. The proposed method was successfully applied to real aquatic products.

引用

页码：19 / 24

页数：6

共 46 条

[1] Molecularly imprinted polymers for bisphenol A for HPLC and SPE from water and milk [J].

Alexiadou, Despina K. ;

Maragou, Niki C. ;

Thomaidis, Nikolaos S. ;

Theodoridis, Georgios A. ;

Koupparis, Michael A. .

JOURNAL OF SEPARATION SCIENCE, 2008, 31 (12) :2272-2282

[2]

[Anonymous], ENV HLTH PERSPECT

[3]

[Anonymous], 2014, BISPH A BPA US FOOD

[4] Determination of bisphenol A in canned foods by immunoaffinity chromatography, HPLC, and fluorescence detection [J].

Braunrath, R ;

Podlipna, D ;

Padlesak, S ;

Cichna-Markl, M .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (23) :8911-8917

[5] Determination of bisphenol A in wine by sol-gel immunoaffinity chromatography, HPLC and fluorescence detection [J].

Brenn-Struckhofova, Z. ;

Cichna-Markl, M. .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2006, 23 (11) :1227-1235

[6] Bisphenol A and Three Other Bisphenol Analogues in Canned Fish Products from the Canadian Market 2014 [J].

Cao, Xu-Liang ;

Popovic, Svetlana .

JOURNAL OF FOOD PROTECTION, 2015, 78 (07) :1402-1407

[7] Exposure to the BPA-Substitute Bisphenol S Causes Unique Alterations of Germline Function [J].

Chen, Yichang ;

Shu, Le ;

Qiu, Zhiqun ;

Lee, Dong Yeon ;

Settle, Sara J. ;

Hee, Shane Que ;

Telesca, Donatello ;

Yang, Xia ;

Allard, Patrick .

PLOS GENETICS, 2016, 12 (07)

[8]

CHENG Y, 1973, BIOCHEM PHARMACOL, V22, P3099

[9] A Review on the Effects of Bisphenol A and Its Derivatives on Skeletal Health [J].

Chin, Kok-Yong ;

Pang, Kok-Lun ;

Mark-Lee, Wun Fui .

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, 2018, 15 (10) :1043-1050

[10] Sample clean-up by sol-gel immunoaffinity chromatography for the determination of bisphenol A in food and urine [J].

Cichna-Markl, Margit .

METHODS, 2012, 56 (02) :186-191

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