Targeted mutagenesis of Drosophila RNaseZ gene by homologous recombination

被引：3

作者：

Andreenkov, O. V. ^{[1
]}

Volkova, E. I. ^{[1
]}

Demakov, S. A. ^{[1
]}

Xie, X. ^{[2
]}

Dubrovsky, E. B. ^{[2
]}

Zhimulev, I. F. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Mol & Cellular Biol, Siberian Branch, Pr Akad Lavrenteva 8, Novosibirsk 2630090, Russia

[2] Fordham Univ, New York, NY 10023 USA

来源：

DOKLADY BIOCHEMISTRY AND BIOPHYSICS | 2016年 / 471卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

CANCER SUSCEPTIBILITY GENE; EFFICIENT;

D O I：

10.1134/S1607672916060065

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For the first time we used a homologous recombination method to obtain complete and precise deletion of Drosophila dRNaseZ gene. In the founder line of flies in which the RNaseZ sequence was replaced by attP site, the full-length sequence of the gene was reintegrated, and its functionality was shown. This approach will allow us to generate further gene mutations in different domains of dRNaseZ protein and discover a broad spectrum and uncover functions outside of tRNA processing.

引用

页码：399 / 402

页数：4

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