The effect of induced anti-follicle-stimulating hormone autoantibody on serum hormone level and apoptosis in rat testis

被引：11

作者：

Yao, Bing ^{[1
]}

Yi, Nan ^{[1
]}

Zhou, Shuigen ^{[2
]}

OuYang, Weiming ^{[3
,4
,5
]}

Xu, Huiru ^{[1
]}

Ge, Yifeng ^{[1
]}

Cui, Yingxia ^{[1
]}

Xia, Xinyi ^{[1
]}

机构：

[1] Nanjing Univ, Inst Clin Lab Med, Nanjing Jinling Hosp, Clin Sch Med Coll, Nanjing 210002, Jiangsu, Peoples R China

[2] Nanjing Univ, Dept Urol, Nanjing Jinling Hosp, Clin Sch Med Coll, Nanjing 210002, Jiangsu, Peoples R China

[3] Yale Univ, Sch Med, Dept Immunobiol, New Haven, CT USA

[4] Yale Univ, Sch Med, Dept Vasc Biol, New Haven, CT USA

[5] Yale Univ, Sch Med, Therapeut Program, New Haven, CT USA

来源：

LIFE SCIENCES | 2012年 / 91卷 / 3-4期

关键词：

Follicle-stimulating hormone; Inhibin B; Testis; FSH autoantibody; Apoptosis; GERM-CELL APOPTOSIS; SERTOLI-CELL; INHIBIN-B; ADULT RATS; NORMAL MEN; HPG MICE; FSH; SPERMATOGENESIS; PATHWAYS; TESTOSTERONE;

D O I：

10.1016/j.lfs.2012.04.026

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aims: Anti-follicle-stimulating hormone (FSH) autoantibody was found to highly correlate with oligospermia and asthenospermia, but the actual effect of FSH autoantibody on spermatogenesis is still unknown. Main methods: In this study, 21-day rats were immunized seven times with FSH peptides linked with Keyhole Limpet Hemocyanin (KLH) (experimental group) or KLH (control group) every 2 weeks. Luteinizing hormone (LH) and inhibits B level in the immunized rat sera were measured by enzyme-linked immunosorbent assay (ELISA). Apoptosis of spermatogenic cells in the testis was detected by in situ end labeling method (TUNEL), and the mRNAs of Box, Bcl-2 and Caspase-3 in testis were detected by fluorescent Quantitative PCR. Key findings: Compared with the control, serum inhibin B level was significantly decreased at all time points (34.49%, 23.20%, and 37.00%) (p <0.05). There was no difference in the serum LH level between experimental and control groups. FSH peptide immunization increased the apoptosis of spermatogenic cells in the testis that was associated with an imbalance of Box and Bcl-2 expression and upregulation of Caspase-3. Significance: These results suggest that FSH autoantibody could cause the reduction of inhibin B, thereby inducing hypospermatogenesis via augment of spermatogenic cell apoptosis. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：83 / 88

页数：6

共 33 条

[1] Serum inhibin B levels reflect sertoli cell function in normal men and men with testicular dysfunction [J].