In vitro hydrolytic degradation of poly(ε-caprolactone) grafted dextran fibers and films

We studied the hydrolytic degradation of poly(epsilon-caprolactone) grafted dextran (PGD) fibers and films (matrices) prepared by electrospinning and solvent evaporation methods, respectively. In vitro degradation and erosion experiments were carried out in phosphate buffered saline (pH 7.4 +/- 0.1) at 37 +/- 1 degrees C for 150 days. Changes in molecular weights and morphologies of the PGD matrices were monitored as a function of degradation time. The extent of degradation was measured by physical weight loss, scanning electron microscopic (SEM) observations, Fourier transform-infrared (FT-IR) spectroscopy, gel permeation chromatography (GPC) and differential scanning calorimetry (DSC). During the progress of hydrolysis, GPC chromatograms appeared bi modal for fibers and bi and trimodal for the films. The crystallization temperature (T-c) and heat of fusion were significantly increased in both matrices; this indicated preferential hydrolytic degradation in amorphous regions followed by cleavage-induced crystallization. The biodegradation rates were faster for the films (28%) than fibers (23%). After 150 days of degradation, the pH was steady at 5.8 +/- 0.3 for fibers and 6.1 +/- 0.3 for films. The faster degradation of the films could be probably due to autocatalysis in the interior of the films and the degraded oligomers are hard to diffuse out into the surrounding solution due to its compact physical geometry. Thus, our preliminary results about the degradation of matrices suggested that PGD nanofibers could be excellent matrices in tissue engineering over the films. (C) 2008 Elsevier Ltd. All rights reserved.