Nanoroughened Surfaces for Efficient Capture of Circulating Tumor Cells without Using Capture Antibodies

被引：154

作者：

Chen, Weiqiang ^{[1
,2
]}

Weng, Shinuo ^{[1
,2
]}

Zhang, Feng ^{[1
,8
]}

Allen, Steven ^{[3
]}

Li, Xiang ^{[1
,2
]}

Bao, Liwei ^{[4
]}

Lam, Raymond H. W. ^{[9
]}

Macoska, Jill A. ^{[5
,6
]}

Merajver, Sofia D. ^{[4
,6
]}

Fu, Jianping ^{[1
,2
,7
]}

机构：

[1] Univ Michigan, Integrated Biosyst & Biomech Lab, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Dept Cellular & Mol Biol, Ann Arbor, MI 48109 USA

[4] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Dept Urol, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA

[7] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[8] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200030, Peoples R China

[9] City Univ Hong Kong, Dept Mech & Biomed Engn, Hong Kong, Hong Kong, Peoples R China

来源：

ACS NANO | 2013年 / 7卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

cancer; circulating tumor cell; nanotopography; biomaterials; microfabrication; CANCER-PATIENTS; LABEL-FREE; EPITHELIAL-CELLS; ADHESION; BLOOD; MICROFLUIDICS; HETEROGENEITY; PERSPECTIVE; PROGRESSION; ENRICHMENT;

D O I：

10.1021/nn304719q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Circulating tumor cells (CTCs) detached from both primary and metastatic lesions represent a potential alternative to invasive biopsies as a source of tumor tissue for the detection, characterization and monitoring of cancers. Here we report a simple yet effective strategy for capturing CTCs without using capture antibodies. Our method uniquely utilized the differential adhesion preference of cancer cells to nanorough surfaces when compared to normal blood cells and thus did not depend on their physical size or surface protein expression, a significant advantage as compared to other existing CTC capture techniques.

引用

页码：566 / 575

页数：10

共 32 条

[1] Highly efficient circulating tumor cell isolation from whole blood and label-free enumeration using polymer-based microfluidics with an integrated conductivity sensor [J].