Cell-Imprinted Substrates Direct the Fate of Stem Cells

被引：100

作者：

Mahmoudi, Morteza ^{[1
,2
,3
]}

Bonakdar, Shahin ^{[1
]}

Shokrgozar, Mohammad A. ^{[1
]}

Aghaverdi, Haniyeh ^{[2
,3
]}

Hartmann, Raimo ^{[4
]}

Pick, Andre ^{[4
]}

Witte, Gregor ^{[4
]}

Parak, Wolfgang J. ^{[4
,5
]}

机构：

[1] Pasteur Inst Iran, Natl Cell Bank, Tehran 13164, Iran

[2] Univ Tehran Med Sci, Fac Pharm, Dept Nanotechnol, Lab NanoBio Interact, Tehran 13164, Iran

[3] Univ Tehran Med Sci, Fac Pharm, Nanotechnol Res Ctr, Tehran 13164, Iran

[4] Univ Marburg, Fachbereich Phys, D-35037 Marburg, Germany

[5] CIC Biomagune, San Sebastian 20009, Spain

来源：

ACS NANO | 2013年 / 7卷 / 10期

关键词：

stem cells; biomimicking; cell morphology; cell fate; differentiation; smart substrates; GROWTH-FACTOR; DIFFERENTIATION; NANOSCALE; BIOMATERIALS; MATRIX; SURFACE; NICHE;

D O I：

10.1021/nn403844q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Smart nanoenvironments were obtained by cell-imprinted substrates based on mature and dedifferentiated chondrocytes as templates. Rabbit adipose derived mesenchymal stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape (as determined in terms of cell morphology) and molecular characteristics (as determined In terms of gene expression) of the cell types which had been used as template for the cell-imprinting. This method might pave the way for a reliable, efficient, and cheap way of controlling stem cell differentiation. Data also suggest that besides residual cellular fragments, which are presented on the template surface, the imprinted topography of the templates plays a role in the differentiation of the stem cells.

引用

页码：8379 / 8384

页数：6

共 42 条

[21] Engineering systems for the generation of patterned co-cultures for controlling cell-cell interactions
Kaji, Hirokazu
Camci-Unal, Gulden
Langer, Robert
Khademhosseini, Ali
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2011, 1810 (03): : 239 - 250
[22] Microscale technologies for tissue engineering and biology
Khademhosseini, A
Langer, R
Borenstein, J
Vacanti, JP
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (08) : 2480 - 2487
[23] Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates
Khademhosseini, Ali
Ferreira, Lino
Blumling, James, III
Yeh, Judy
Karp, Jeffrey M.
Fukuda, Junji
Langer, Robert
[J]. BIOMATERIALS, 2006, 27 (36) : 5968 - 5977
[24] Growth factor delivery-based tissue engineering: general approaches and a review of recent developments
Lee, Kangwon
Silva, Eduardo A.
Mooney, David J.
[J]. JOURNAL OF THE ROYAL SOCIETY INTERFACE, 2011, 8 (55) : 153 - 170
[25] Geometric Control of Stem Cell Differentiation Rate on Surfaces
Luo, Wei
Jones, Stephanie R.
Yousaf, Muhammad N.
[J]. LANGMUIR, 2008, 24 (21) : 12129 - 12133
[26] McMurray RJ, 2011, NAT MATER, V10, P637, DOI [10.1038/NMAT3058, 10.1038/nmat3058]
[27] Mei Y, 2010, NAT MATER, V9, P768, DOI [10.1038/NMAT2812, 10.1038/nmat2812]
[28] Murtuza B, 2009, TISSUE ENG PART B-RE, V15, P443, DOI [10.1089/ten.teb.2009.0006, 10.1089/ten.TEB.2009.0006]
[29] Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Ott, Harald C.
Matthiesen, Thomas S.
Goh, Saik-Kia
Black, Lauren D.
Kren, Stefan M.
Netoff, Theoden I.
Taylor, Doris A.
[J]. NATURE MEDICINE, 2008, 14 (02) : 213 - 221
[30] InfoBiology by printed arrays of microorganism colonies for timed and on-demand release of messages
Palacios, Manuel A.
Benito-Pena, Elena
Manesse, Mael
Mazzeo, Aaron D.
LaFratta, Christopher N.
Whitesides, George M.
Walt, David R.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (40) : 16510 - 16514

← 1 2 3 4 5 →