Fractionation of hydrolysates from concentrated lecithin free egg yolk protein dispersions by ultrafiltration

被引：11

作者：

Penaranda-Lopez, A. L. ^{[1
]}

Brito-de la Fuente, Edmundo ^{[2
]}

Torrestiana-Sanchez, Beatriz ^{[1
]}

机构：

[1] Tecnol Nacl Mexico IT Veracruz, Av MA De Quevedo 2779, Veracruz, Veracruz, Mexico

[2] Fresenius Kabi Deutschland GmbH, Pharmaceut Div, Prod & Proc Engn Ctr, Global Pharmaceut, Siemenstr 27, D-61352 Bad Homburg, Germany

来源：

FOOD AND BIOPRODUCTS PROCESSING | 2020年 / 123卷

关键词：

Lecithin-free egg yolk hydrolysates; Ultrafiltration; Diafiltration; Sustainable flux; Functional properties; NANOFILTRATION IMPACT; BY-PRODUCT; ANTIOXIDANT; PEPTIDES; OXIDATION;

D O I：

10.1016/j.fbp.2020.07.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A membrane process for the recovery of >= 90 % of the soluble peptides (with antioxidant activity) found in concentrated lecithin-free egg yolk hydrolysates (LFEYH) was established. Hydrolysis of concentrated free lecithin egg yolk protein dispersions (10 and 20 %) was conducted with Alcalase 2.4L, an endoprotease from Bacillus Licheniformis. The obtained hydrolysates were fractionated by ultrafiltration (UF), where the effect of the membrane material and molecular weight cut-off (MWCO) was studied. Two flat sheet regenerated cellulose membranes and three tubular ceramic membranes with different MWCOs (50, 10 and 5 kDa) were tested. Different hydrodynamic operating conditions were established in all membranes to work under sustainable flux during UF. The 50 kDa ceramic membrane was found to be the most efficient in terms of flux (78.45 and 47.84 kg m(-2) h(-1) for 10 and 20 % protein dispersions, respectively) and showed lower fouling. The use of diafiltration (DF), allowed recovering more than 90 % of soluble and heat-stable peptides, which also showed high antioxidant activity, from hydrolysates produced in both concentrated (10 and 20 %) egg yolk protein dispersions. (C) 2020 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

引用

页码：209 / 216

页数：8

共 18 条

[1]

Adler-Nielsen J., 1986, ENZYMIC HYDROLYSIS F

[2] Antioxidant properties of Australian canola meal protein hydrolysates [J].

Alashi, Adeola M. ;

Blanchard, Christopher L. ;

Mailer, Rodney J. ;

Agboola, Samson O. ;

Mawson, A. John ;

He, Rong ;

Girgih, Abraham ;

Aluko, Rotimi E. .

FOOD CHEMISTRY, 2014, 146 :500-506

[3] Critical and sustainable fluxes: Theory, experiments and applications [J].

Bacchin, P. ;

Aimar, P. ;

Field, R. W. .

JOURNAL OF MEMBRANE SCIENCE, 2006, 281 (1-2) :42-69

[4] Fractionation of fish protein hydrolysates by ultrafiltration and nanofiltration: impact on peptidic populations [J].

Bourseau, P. ;

Vandanjon, L. ;

Jaouen, P. ;

Chaplain-Derouiniot, M. ;

Masse, A. ;

Guerard, F. ;

Chabeaud, A. ;

Fouchereau-Peron, M. ;

Le Gal, Y. ;

Ravallec-Ple, R. ;

Berge, J. -P. ;

Picot, L. ;

Piot, J. -M. ;

Batista, I. ;

Thorkelsson, G. ;

Delannoy, C. ;

Jakobsen, G. ;

Johansson, I. .

DESALINATION, 2009, 244 (1-3) :303-320

[5] Effects of Ionic Strength on the Enzymatic Hydrolysis of Diluted and Concentrated Whey Protein Isolate [J].

Butre, Claire I. ;

Wierenga, Peter A. ;

Gruppen, Harry .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (22) :5644-5651

[6] Fractionation by ultrafiltration of a saithe protein hydrolysate (Pollachius virens): Effect of material and molecular weight cut-off on the membrane performances [J].

Chabeaud, A. ;

Vandanjon, L. ;

Bourseau, P. ;

Jaouen, P. ;

Guerard, F. .

JOURNAL OF FOOD ENGINEERING, 2009, 91 (03) :408-414

[7]

Cheryan M., 1998, ULTRAFILTRATION MICR, P293

[8] On the actual cleanability of polyethersulfone membrane fouled by proteins at critical or limiting flux [J].

Diagne, Ndeye Wemsy ;

Rabiller-Baudry, Murielle ;

Paugam, Lydie .

JOURNAL OF MEMBRANE SCIENCE, 2013, 425 :40-47

[9] Egg-yolk protein by-product as a source of ACE-inhibitory peptides obtained with using unconventional proteinase from Asian pumpkin (Cucurbita ficifolia) [J].

Eckert, Ewelina ;

Zambrowicz, Aleksandra ;

Pokora, Marta ;

Setner, Bartosz ;

Dabrowska, Anna ;

Szoltysik, Marek ;

Szewczuk, Zbigniew ;

Polanowski, Antoni ;

Trziszka, Tadeusz ;

Chrzanowska, Jozefa .

JOURNAL OF PROTEOMICS, 2014, 110 :107-116

[10] Economic aspects of critical flux operability in star shaped microfiltration membranes: Influence of some operating conditions [J].

Garcia, F. J. Garcia ;

Chiu, T. Y. .

JOURNAL OF MEMBRANE SCIENCE, 2008, 325 (02) :641-646

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