PrPsc is not detected in peripheral blood leukocytes of scrapie-infected sheep: Determining the limit of sensitivity by immunohistochemistry

被引：17

作者：

Herrmann, LM ^{[1
]}

Baszler, TV

Knowles, DP

Cheevers, WP

机构：

[1] ARS, USDA, ADRU, ADBF 3003, Pullman, WA 99164 USA

[2] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2002年 / 9卷 / 02期

关键词：

D O I：

10.1128/CDLI.9.2.499-502.2002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrPSc by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrPSc-positive cells were detected in 2.05% 0.28% of 3 x 10(6) DRLN cells, but were not detected in 3 x 10(6) PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrPSc-positive cells in 3 x 10(6) PBLs.

引用

页码：499 / 502

页数：4

共 17 条

[1] IDENTIFICATION OF A PROTEIN THAT PURIFIES WITH THE SCRAPIE PRION [J].