Response to metal stress of Nicotiana langsdorffii plants wild-type and transgenic for the rat glucocorticoid receptor gene

被引：20

作者：

Fuoco, Roger ^{[1
]}

Bogani, Patrizia ^{[2
]}

Capodaglio, Gabriele ^{[3
]}

Del Bubba, Massimo ^{[4
]}

Abollino, Ornella ^{[5
]}

Giannarelli, Stefania ^{[1
]}

Spiriti, Maria Michela ^{[2
]}

Muscatello, Beatrice ^{[1
]}

Doumett, Saer ^{[4
]}

Turetta, Clara ^{[6
]}

Zangrando, Roberta ^{[6
]}

Zelano, Vincenzo ^{[5
]}

Buiatti, Marcello ^{[2
]}

机构：

[1] Univ Pisa, Dept Chem & Ind Chem, I-56126 Pisa, Italy

[2] Univ Florence, Dept Biol, I-50019 Sesto Fiorentino 10, FI, Italy

[3] Univ Ca Foscari, Dept Environm Sci Informat & Stat, I-30121 Venice, Italy

[4] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, FI, Italy

[5] Univ Turin, Dept Chem, I-10125 Turin, Italy

[6] CNR, Inst Dynam Environm Proc, I-30123 Venice, Italy

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2013年 / 170卷 / 07期

关键词：

Transgenic N. langsdorffii; Metal stress; Phytohormones; Metabolomics; STEROID-BINDING PROTEIN-1; ABSCISIC-ACID; OXIDATIVE STRESS; ABIOTIC STRESS; CADMIUM; CHROMIUM; ARABIDOPSIS; TOLERANCE; GROWTH; AUXIN;

D O I：

10.1016/j.jplph.2012.12.009

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Recently our findings have shown that the integration of the gene coding for the rat gluco-corticoid receptor (GR receptor) in Nicotiana langsdorffii plants induced morphophysiological effects in transgenic plants through the modification of their hormonal pattern. Phytohormones play a key role in plant responses to many different biotic and abiotic stresses since a modified hormonal profile up-regulates the activation of secondary metabolites involved in the response to stress. In this work transgenic GR plants and isogenic wild type genotypes were exposed to metal stress by treating them with 30 ppm cadmium(II) or 50 ppm chromium(VI). Hormonal patterns along with changes in key response related metabolites were then monitored and compared. Heavy metal up-take was found to be lower in the GR plants. The transgenic plants exhibited higher values of S-abscisic acid (S-ABA) and 3-indole acetic acid (IAA), salicylic acid and total polyphenols, chlorogenic acid and antiradical activity, compared to the untransformed wild type plants. Both Cd and Cr treatments led to an increase in hormone concentrations and secondary metabolites only in wild type plants. Analysis of the results suggests that the stress responses due to changes in the plant's hormonal system may derive from the interaction between the GR receptor and phytosteroids, which are known to play a key role in plant physiology and development. (C) 2013 Elsevier GmbH. All rights reserved.

引用

页码：668 / 675

页数：8

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