A fluorous capping strategy for Fmoc-based automated and manual solid-phase peptide synthesis

被引：19
作者：
Montanari, V
Kumar, K [1 ]
机构：
[1] Tufts Univ, Dept Chem, Medford, MA 02155 USA
[2] Tufts Univ, New England Med Ctr, Ctr Canc, Boston, MA 02110 USA
来源：
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY | 2006年 / 2006卷 / 04期
关键词：
protein design; peptide synthesis; organic methodology; fluorous chemistry; automated peptide synthesis; protein engineering;
D O I：
10.1002/ejoc.200500958
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Just add water: Peptides synthesized by the use of standardized Fmoc protocols with commercial automated synthesizers can be purified from deletion products by simple centrifugation of aqueous solutions. The deletion products are capped with fluorous trivalent iodonium salts. At the end of the synthesis, the crude peptide is dissolved in water and centrifuged, and the deletion products precipitate leaving only the full length peptide in solution. Protocols for generalized use of this strategy are reported. (c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006.
引用
收藏
页码：874 / 877
页数：4
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