The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings

被引：54
作者：
Liu, Jun [1 ]
Wang, Xiaodong [1 ,2 ]
Jin, Qiming [3 ]
Jin, Taocong [1 ]
Chang, Syweren [1 ]
Zhang, Zhaocheng [1 ]
Czajka-Jakubowska, Agata [4 ]
Giannobile, William V. [3 ]
Noer, Jacques E. [1 ]
Clarkson, Brian H. [1 ]
机构：
[1] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA
[2] Fourth Mil Med Univ, Sch Stomatol, Dept Operat Dent & Endodont, Xian 710032, Shaanxi, Peoples R China
[3] Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
[4] Poznan Univ Med Sci, Dept Conservat Dent & Periodontol, Poznan, Poland
来源：
BIOMATERIALS | 2012年 / 33卷 / 20期
关键词：
Fluorapatite; Differentiation; Mineralization; Gene profile; Stem cells; EPIDERMAL-GROWTH-FACTOR; GENE-EXPRESSION; BONE; IMPLANTS; MSX1; OSTEOMALACIA; OSTEOBLASTS; ADHESION; SERUM; DMP1;
D O I：
10.1016/j.biomaterials.2012.03.050
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
In this study, the effect of ordered rod-like FA coatings of metal discs on adipose-derived stem cell (ASC)'s growth, differentiation and mineralization was studied in vitro; and their mineral inductive effects in vivo. After 3 and 7 days, the cell number on the metal surfaces was significantly higher than those on the ordered and disordered FA surfaces. However, after 4 weeks much greater amounts of mineral formation was induced on the two FA surfaces with and even without osteogenesis induction. The osteogenic profiles showed the up regulation of a set of pro-osteogenic transcripts and bone mineralization phenotypic markers when the ASCs were grown on FA surfaces compared to metal surfaces at 7 and 21 days. In addition to BMP and TGF beta signaling pathways, EGF and FGF pathways also appeared to be involved in ASC differentiation and mineralization. In vivo studies showed accelerated and enhanced mineralized tissue formation integrated within ordered FA coatings. After 5 weeks, over 80% of the ordered FA coating was integrated with a mineralized tissue layer covering the implants. Both the intrinsic properties of the FA crystals and the topography of the FA coating appeared to dominate the cell differentiation and mineralization process. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：5036 / 5046
页数：11
相关论文
共 38 条
  • [1] Locally Applied Vascular Endothelial Growth Factor A Increases the Osteogenic Healing Capacity of Human Adipose-Derived Stem Cells by Promoting Osteogenic and Endothelial Differentiation
    Behr, Bjoern
    Tang, Chad
    Germann, Guenter
    Longaker, Michael T.
    Quarto, Natalina
    [J]. STEM CELLS, 2011, 29 (02) : 286 - 296
  • [2] Influence of fluorapatite on the properties of thermally sprayed hydroxyapatite coatings
    Bhadang, KA
    Gross, KA
    [J]. BIOMATERIALS, 2004, 25 (20) : 4935 - 4945
  • [3] Bieback K, 2010, TISSUE ENG PT A, V16, P3467, DOI [10.1089/ten.tea.2009.0727, 10.1089/ten.TEA.2009.0727]
  • [4] Differential temporal expression of members of the transforming growth factor β superfamily during murine fracture healing
    Cho, TJ
    Gerstenfeld, LC
    Einhorn, TA
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2002, 17 (03) : 513 - 520
  • [5] Msx1 and Dlx5 Function Synergistically to Regulate Frontal Bone Development
    Chung, Il-Hyuk
    Han, Jun
    Iwata, Junichi
    Chai, Yang
    [J]. GENESIS, 2010, 48 (11) : 645 - 655
  • [6] DeLuca S, 2006, INT J PROSTHODONT, V19, P491
  • [7] A MECHANICAL INVESTIGATION OF FLUORAPATITE, MAGNESIUMWHITLOCKITE, AND HYDROXYLAPATITE PLASMA-SPRAYED COATINGS IN GOATS
    DHERT, WJA
    KLEIN, CPAT
    WOLKE, JGC
    VANDERVELDE, EA
    DEGROOT, K
    ROZING, PM
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1991, 25 (10): : 1183 - 1200
  • [8] Biological Performance of Chemical Hydroxyapatite Coating Associated With Implant Surface Modification by Laser Beam: Biomechanical Study in Rabbit Tibias
    Faeda, Rafael S.
    Tavares, Hewerson S.
    Sartori, Rafael
    Guastaldi, Antonio C.
    Marcantonio, Elcio, Jr.
    [J]. JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2009, 67 (08) : 1706 - 1715
  • [9] Farré-Guasch E, 2010, TISSUE ENG PART C-ME, V16, P1083, DOI [10.1089/ten.tec.2009.0487, 10.1089/ten.TEC.2009.0487]
  • [10] Dissolution behavior of plasma-sprayed hydroxyapatite coatings
    Fazan, F
    Marquis, PM
    [J]. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2000, 11 (12) : 787 - 792
1234