Comparative metabolic profiling of cultivated and wild black soybeans reveals distinct metabolic alterations associated with their domestication

被引:20
作者
Hyeon, Hyejin [1 ]
Xu, Jiu Liang [2 ,3 ,4 ,5 ]
Kim, Jae Kwang [1 ]
Choi, Yongsoo [2 ,6 ]
机构
[1] Incheon Natl Univ, Div Life Sci, Coll Life Sci & Bioengn, Incheon 22012, South Korea
[2] Korea Inst Sci & Technol KIST, Nat Prod Res Ctr, Kangnung 25451, South Korea
[3] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China
[4] China Agr Univ, Natl Acad Agr Green Dev, Beijing 100193, Peoples R China
[5] China Agr Univ, Sch Agr Green Dev, Beijing 100193, Peoples R China
[6] Univ Sci & Technol, Dept Biol Chem, Daejeon 305350, South Korea
关键词
Metabolic profiling; Cultivated black soybean; Wild black soybean; Multivariate analysis; Primary metabolites; Secondary metabolites; RICE ORYZA-SATIVA; GLYCINE-MAX; ANTIOXIDANT ACTIVITIES; AMINO-ACIDS; SEED COATS; IN-VITRO; ANTHOCYANINS; SOJA; PROLIFERATION; ISOFLAVONES;
D O I
10.1016/j.foodres.2020.109290
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Generally, cultivated black soybean (CBS) has been used as a major source of various nutrients for humans and animals. To assess the metabolic alterations induced by domestication in soybean, we performed a comprehensive metabolite profiling of 56 soybean varieties, including 28 CBS and 28 wild black soybean (WBS) varieties. A total of 48 metabolites were characterized, including 45 primary and 3 secondary metabolites, from CBS and WBS. The results of principal component analysis and hierarchical cluster analysis (HCA) revealed significant metabolic differences between CBS and WBS that were closely related to metabolic pathways. The results indicate that flavonoids correlated positively with phenylalanine, a precursor for phenylpropanoid biosynthesis; the contents of flavonoids and phenylpropanoids were higher in WBS. Pathway analysis revealed that CBS contained large amounts of TCA cycle intermediates, amino acids, and fatty acids as a result of increased energy metabolism, amino acid metabolism, and seed filling. The projection to latent structure method, using the partial least squares method, was applied to predict the flavonoid content in soybean seed, which indicated that sucrose, threonic acid, citric acid, and fatty acids are important in predicting the antioxidant content of samples. This work will provide important information for designing new soybean cultivars with enhanced nutritional and agricultural traits.
引用
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页数:10
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