1H, 13C, and 15N backbone assignments of the C-terminal region of the human retinoic acid-induced protein 2

被引：4

作者：

Lang, Andras ^{[1
]}

Goradia, Nishit ^{[2
]}

Wikman, Harriet ^{[3
]}

Werner, Stefan ^{[3
]}

Wilmanns, Matthias ^{[2
,4
]}

Ohlenschlaeger, Oliver ^{[1
]}

机构：

[1] Leibniz Inst Aging, Fritz Lipmann Inst, Beutenbergstr 11, D-07745 Jena, Germany

[2] European Mol Biol Lab, Hamburg Unit, Notkestr 85, D-22607 Hamburg, Germany

[3] Univ Med Ctr Hamburg Eppendorf, Inst Tumor Biol, Martinistr 52, D-20246 Hamburg, Germany

[4] Univ Med Ctr Hamburg Eppendorf, Martinistr 52, D-20246 Hamburg, Germany

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2020年 / 14卷 / 02期

关键词：

Resonance assignments; Chemical shift; Heteronuclear NMR; Retinoic acid-induced protein 2; Tumor suppressor; NMR-SPECTROSCOPY;

D O I：

10.1007/s12104-020-09960-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Retinoic acid-induced protein 2 is a human protein of 530 residues encoded by the RAI2 gene (Q9Y5P3; RAI2_HUMAN). RAI2 is a novel tumor suppressor protein whose depletion in breast cancer cell lines results in the downregulation of several genes associated with differentiation along with increased invasiveness and aggressive tumor phenotype of the cells. The role of the protein is specified to be a transcriptional regulator that promotes chromosomal stability and hence controls the expression of several regulators of cancer and metastasis. Structurally, RAI2 remains an unknown entity and, hence, to obtain a detailed view on the structure function relationship we report the(1)H,C-13, and(15)N resonance assignments for the backbone and side chain nuclei of the C-terminal region (a.a. 303-451 of UniProt Q9Y5P3) of RAI2.

引用

页码：271 / 275

页数：5

共 16 条

[1]

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[2] (H)N(COCA)NH and (HN)under-bar(COCA)NH experiments for H-1-N-15 backbone assignments in C-13/N-15-labeled proteins [J].