DNA ploidy assessment method applied to primary breast carcinoma FNA biopsies

被引：0

作者：

Czuba, A ^{[1
]}

Lange, D ^{[1
]}

Chmielik, E ^{[1
]}

Woloszynska, K ^{[1
]}

Wojciechowska, E ^{[1
]}

Snietura, M ^{[1
]}

Liszka, J ^{[1
]}

机构：

[1] Marie Curie Sklodowska Curie Mem Inst, Ctr Oncol, Dept Pathol, PL-44100 Gliwice, Poland

来源：

FOLIA HISTOCHEMICA ET CYTOBIOLOGICA | 2002年 / 40卷 / 02期

关键词：

breast carcinoma; fine-needle aspiration biopsy; DNA ploidy; flow cytometry; immunocytochemistry;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The goal of our study was to assess suitability of FNA biopsy material as a source of samples (cell suspension) for DNA ploidy assessment in neoplastic tumors using flow cytometry, DNA ploidy is an established prognostic factor in many types of cancers. Aneuploid breast tumors are characterized by increased aggressiveness which manifests itself through rapid local progression and metastatic spread. Investigated specimens were breast cancer FNA biopsy cell suspensions. Measurements were performed using flow cytometry. Material studied comprised 143 cases analyzed in 1999-2000. We found in this group 101 carcinoma cases with aneuploid type and 42 cases of primary breast carcinoma with diploid type of cell cycle. Immunocytochemical assesssment of estrogen receptor and progesterone receptor status was performed in group of 105 cases. DNA ploidy was compared to receptor status of the investigated cells. DNA aneuploidy correlated with weak or no reaction for the presence of estrogen and progesterone receptors. Our study demonstrates the suitability of DNA ploidy assessment method applied to cytological material from FNA biopsies.

引用

页码：105 / 106

页数：2

共 7 条

[1]

BOZETTI C, 1994, BREAST CANC RES TREA, V32, P221

[2]

FRANZEN S, 1968, ACTA RADIOL THER PHY, V7, P241

[3]

KLINE TS, 1979, CANCER, V44, P1458, DOI 10.1002/1097-0142(197910)44:4<1458::AID-CNCR2820440440>3.0.CO

[4]

2-T

[5]

LJUNG BM, 1994, CANCER, V74, P1000, DOI 10.1002/1097-0142(19940801)74:3+<1000::AID-CNCR2820741505>3.0.CO

[6]

2-F

[7] THE ASSESSMENT OF MULTIPLE-VARIABLES ON BREAST-CARCINOMA FINE-NEEDLE ASPIRATION (FNA) CYTOLOGY SPECIMENS - METHOD, PRELIMINARY-RESULTS AND PROGNOSTIC ASSOCIATIONS [J].

PINDER, SE ;

WENCYK, PM ;

NAYLOR, HE ;

BELL, JA ;

ELSTON, CW ;

ROBERTSON, JFR ;

BLAMEY, RW ;

ELLIS, IO .

CYTOPATHOLOGY, 1995, 6 (05) :316-324

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