Detection of Reactive Oxygen Species by a Carbon-Dot-Ascorbic Acid Hydrogel

被引：59

作者：

Bhattacharya, Sagarika ^{[1
]}

Sarkar, Rhitajit ^{[3
]}

Nandi, Sukhendu ^{[1
,4
]}

Porgador, Angel ^{[3
]}

Jelinek, Raz ^{[1
,2
]}

机构：

[1] Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Ilse Katz Inst Nanotechnol, IL-84105 Beer Sheva, Israel

[3] Ben Gurion Univ Negev, Fac Hlth Sci, Shraga Segal Dept Microbiol Immunol & Genet, IL-84105 Beer Sheva, Israel

[4] Ruhr Univ Bochum, Univ Str 150, D-44780 Bochum, Germany

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 01期

关键词：

SEMICONDUCTING POLYMER NANOPARTICLES; SUPEROXIDE ANION; DRUG-DELIVERY; CANCER-CELLS; FLUORESCENCE; LIVE; METAL; AMPHIPHILES; MECHANISMS; TOXICITY;

D O I：

10.1021/acs.analchem.6b03749

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Detection of reactive oxygen species (ROS) is important in varied biological processes, disease diagnostics, and chemotherapeutic drug screening. We constructed a ROS sensor comprising an ascorbic acid -based hydrogel encapsulating luminescent amphiphilic carbon-dots (C-dots). The sensing mechanism is based upon ROS-induced oxidation of the ascorbic acid units-within the hydrogel scaffold; as a consequence, the hydrogel framework collapses resulting in aggregation of the C-dots and quenching of their luminescence. The C-dot hydrogel platform exhibits high sensitivity and detected ROS generated chemically in solution and in actual cell environments. We demonstrate application of the C-dot hydrogel for evaluating the efficacy of a chemotherapeutic substance, underscoring the potential of the system for drug screening applications.

引用

页码：830 / 836

页数：7

共 69 条

[1] Nanotubes from a vitamin C-based bolaamphiphile [J].