Serum Autoantibodies in Mice Immunized with Syngeneic Testicular Germ Cells Alone

被引：8

作者：

Hirai, Shuichi ^{[1
]}

Naito, Munekazu ^{[1
]}

Terayama, Hayato ^{[1
]}

Hatayama, Naoyuki ^{[1
]}

Qu, Ning ^{[1
]}

Musha, Muhetaerjiang ^{[1
]}

Itoh, Masahiro ^{[1
]}

机构：

[1] Tokyo Med Univ, Dept Anat, Shinjuku Ku, Tokyo 1608402, Japan

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2013年 / 70卷 / 06期

关键词：

Autoantibodies; epididymis; germ cells; mouse; spermatozoa; testis; EXPERIMENTAL AUTOIMMUNE ORCHITIS; MURINE MODEL; TESTIS; AUTOANTIGENS; ANTIBODIES; THYMECTOMY; SPERM; BLOOD;

D O I：

10.1111/aji.12145

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

ProblemThe aim of this study was to identify the characteristics of serum autoantibodies in experimental autoimmune orchitis (EAO) induced by immunization with syngeneic testicular germ cells (TGC) alone in mice. Method of studyThe serum autoantibodies were analyzed by enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunohistochemical staining. ResultsSerum autoantibody titers against TGC and epididymal spermatozoa (ES) gradually increased with the development of EAO. At the initial stage of EAO, serum autoantibodies reacted with only a few antigens of TGC and ES, while in the later stage, the reactions with various antigens of TGC and ES occurred. However, the autoantibodies were specific to the testis and epididymis but not to other organs. ConclusionThese results indicate that titers and kinds of autoantibodies in TGC-immunized mice increase with EAO development and they are specific to TGC and ES.

引用

页码：509 / 517

页数：9

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