Kinetics of in vivo bacteriochlorophyll fluorescence yield and the state of photosynthetic apparatus of purple bacteria

被引：14
作者：
Bina, David [1 ,2 ]
Litvin, Radek [1 ,2 ]
Vacha, Frantisek [1 ,2 ,3 ]
机构：
[1] Biol Ctr AVCR, Inst Plant Mol Biol, VVI, Ceske Budejovice 37005, Czech Republic
[2] Univ S Bohemia, Fac Sci, Ceske Budejovice 37005, Czech Republic
[3] Univ S Bohemia, Inst Phys Biol, Nove Hrady 37333, Czech Republic
来源：
PHOTOSYNTHESIS RESEARCH | 2009年 / 99卷 / 02期
关键词：
Photosynthesis; Fluorescence induction; Rhodobacter sphaeroides; Membrane potential; Light scattering; Absorbance changes; EXCITATION-ENERGY TRANSFER; APPLIED ELECTRIC-FIELDS; CHLOROPHYLL FLUORESCENCE; RHODOBACTER-SPHAEROIDES; RHODOSPIRILLUM-RUBRUM; REACTION CENTERS; QUANTUM YIELD; ANTENNA ORGANIZATION; WHOLE-CELLS; RHODOPSEUDOMONAS SPHEROIDES;
D O I：
10.1007/s11120-009-9408-4
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The light-induced electron transport in purple bacterium Rhodobacter sphaeroides was studied in vivo by means of kinetic difference absorption spectroscopy and kinetics of bacteriochlorophyll fluorescence yield. Measurements of redox state of the oxidised primary donor and cytochrome c and the membrane potential revealed a complex pattern of changes of the electron flow. Effects of the membrane potential on the fluorescence yield were also analysed, and a model for the fluorescence induction curve is presented. The data indicate substantial positive effect of the membrane potential on the fluorescence emission in vivo. Moreover, light-induced changes in light scattering were observed, which suggests occurrence of structural changes on the level of the photosynthetic membrane.
引用
收藏
页码：115 / 125
页数：11
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