TRPV6 is not required for 1α, 25-dihydroxyvitamin D3-induced intestinal calcium absorption in vivo

被引：64

作者：

Kutuzova, Galina D. ^{[1
]}

Sundersingh, Flora ^{[1
]}

Vaughan, Jennifer ^{[1
]}

Tadi, Bulli Padmaja ^{[1
]}

Ansay, Susan E. ^{[1
]}

Christakos, Sylvia ^{[2
]}

DeLuca, Hector F. ^{[1
]}

机构：

[1] Univ Wisconsin Madison, Dept Biochem, Madison, WI 53706 USA

[2] Univ Med & Dent New Jersey, Sch Med, Dept Biochem & Mol Biol, Newark, NJ 07103 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 50期

关键词：

microarrays; TRPV6 KO mice; vitamin D; vitamin D mechanism; calcium channel;

D O I：

10.1073/pnas.0810761105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The requirement for TRPV6 for vitamin D-dependent intestinal calcium absorption in vivo has been examined by using vitamin D-deficient TRPV6 null mice and littermate wild-type mice. Each of the vitamin D-deficient animals received each day for 4 days 50 ng of 1,25-dihydroyvitamin D-3 in 0.1 ml of 95% propylene glycol:5% ethanol vehicle or vehicle only. Both the wild-type and TRPV6 null mice responded equally well to 1,25-dihydroxyvitamin D3 in increasing intestinal calcium absorption. These results, along with our microarray data, demonstrate that TRPV6 is not required for vitamin D-induced intestinal calcium absorption and may not carry out a significant role in this process. These and previous results using calbindin D9k null mutant mice illustrate that molecular events in the intestinal calcium absorption process in response to the active form of vitamin D remain to be defined.

引用

页码：19655 / 19659

页数：5

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