Loop-Mediated Isothermal Amplification for the Event-Specific Detection of Wheat B73-6-1

被引：14
作者：
Cheng, Yang [1 ]
Zhang, Minghui [2 ]
Hu, Kun [1 ]
Sun, Fangda [2 ]
Tao, Ran [3 ]
Gao, Xuejun [2 ]
Luan, Fengxia [1 ]
机构：
[1] Heilongjiang Entry Exit Inspect & Quarantine Bur, Harbin, Peoples R China
[2] Northeast Agr Univ, Res Ctr Life Sci & Biotech, Detecting Ctr Agr Genetically Modified Organism, Harbin, Peoples R China
[3] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100094, Peoples R China
来源：
FOOD ANALYTICAL METHODS | 2014年 / 7卷 / 02期
基金：
中国国家自然科学基金;
关键词：
LAMP; Event-Specific Detection; Wheat B73-6-1; GMO; MYCOBACTERIUM-TUBERCULOSIS COMPLEX; INFECTIOUS-DISEASES; CLINICAL-DIAGNOSIS; RAPID DETECTION; LAMP; DNA; SEQUENCE; ASSAY;
D O I：
10.1007/s12161-013-9718-1
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
To develop effective alternatives for detecting genetically modified organisms, we constructed one loop-mediated isothermal amplification (LAMP) event-specific detection method for wheat B73-6-1 in this study. This event-specific LAMP assay can be performed within 38 min without any PCR equipment and the LAMP products can be directly observed by the naked eye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about six copies of haploid wheat-genomic DNA. Furthermore, the high specificity of LAMP assays was determined. High specificity and sensitivity, cost-effectiveness, and low requirement of equipment of the developed event-specific LAMP assay of B73-6-1 allow this method to be useful in transgenic wheat samples analysis, especially in highly processed products.
引用
收藏
页码：500 / 505
页数：6
相关论文
共 30 条
  • [1] Silencing of HMW glutenins in transgenic wheat expressing extra HMW subunits
    Alvarez, ML
    Guelman, S
    Halford, NG
    Lustig, S
    Reggiardo, MI
    Ryabushkina, N
    Shewry, P
    Stein, J
    Vallejos, RH
    [J]. THEORETICAL AND APPLIED GENETICS, 2000, 100 (02) : 319 - 327
  • [2] Bai Yue Bai Yue, 2009, Journal of Triticeae Crops, V29, P199
  • [3] Transformation of wheat with high molecular weight subunit genes results in improved functional properties
    Barro, F
    Rooke, L
    Békés, F
    Gras, P
    Tatham, AS
    Fido, R
    Lazzeri, PA
    Shewry, PR
    Barceló, P
    [J]. NATURE BIOTECHNOLOGY, 1997, 15 (12) : 1295 - 1299
  • [4] A Rapid Loop-Mediated Isothermal Amplification Assay Targeting hspX for the Detection of Mycobacterium tuberculosis Complex
    Bi, Aixiao
    Nakajima, Chie
    Fukushima, Yukari
    Tamaru, Aki
    Sugawara, Isamu
    Kimura, Akio
    Kawahara, Ryuji
    Hu, Zhongyi
    Suzuki, Yasuhiko
    [J]. JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2012, 65 (03) : 247 - 251
  • [5] Milling and baking properties of field grown wheat expressing HMW subunit transgenes
    Darlington, H
    Fido, R
    Tatham, AS
    Jones, H
    Salmon, SE
    Shewry, PR
    [J]. JOURNAL OF CEREAL SCIENCE, 2003, 38 (03) : 301 - 306
  • [6] Applications of Loop-Mediated Isothermal DNA Amplification
    Fu, Shijun
    Qu, Guanggang
    Guo, Shijin
    Ma, Lin
    Zhang, Na
    Zhang, Songlin
    Gao, Sanyang
    Shen, Zhiqiang
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2011, 163 (07) : 845 - 850
  • [7] Real-time loop-mediated isothermal amplification for the CaMV-35S promoter as a screening method for genetically modified organisms
    Fukuta, S
    Mizukami, Y
    Ishida, A
    Ueda, J
    Hasegawa, M
    Hayashi, I
    Hashimoto, M
    Kanbe, M
    [J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2004, 218 (05) : 496 - 500
  • [8] Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-mediated Isothermal Amplification Method
    Guan, Xiaoyan
    Guo, Jinchao
    Shen, Ping
    Yang, Litao
    Zhang, Dabing
    [J]. FOOD ANALYTICAL METHODS, 2010, 3 (04) : 313 - 320
  • [9] Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis
    Han, Eun-Taek
    Watanabe, Risa
    Sattabongkot, Jetsumon
    Khuntirat, Benjawan
    Sirichaisinthop, Jeeraphat
    Iriko, Hideyuki
    Jin, Ling
    Takeo, Satoru
    Tsuboi, Takafumi
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (08) : 2521 - 2528
  • [10] Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M-avium, and M-intracellulare in sputum samples
    Iwamoto, T
    Sonobe, T
    Hayashi, K
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (06) : 2616 - 2622
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