Molecular association processes and fluorescent characteristics of nanomarkers of the fluorescein family in solutions of bovine serum albumin

被引:3
作者
Vlasova, I. M. [1 ]
Kuleshova, A. A. [2 ]
Vlasov, A. A.
Saletsky, A. M.
机构
[1] Moscow MV Lomonosov State Univ, Fac Phys, Dept Gen Phys, Moscow 119991, Russia
[2] Moscow MV Lomonosov State Univ, Fac Phys, Dept Quantum Theory & High Energy Phys, Moscow 119991, Russia
关键词
fluorescein; eosin; erythrosine; Rose Bengal; bovine serum albumin; nanomarker; RAMAN-SPECTROSCOPY; BINDING; PROBES; EOSIN; MECHANISM;
D O I
10.3103/S0027134913040097
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
An analysis of the molecular association and fluorescent characteristics of nanomarkers of the fluorescein family, viz., fluorescein, erythrosine, eosine, and Rose Bengal, in BSA solutions was conducted. For all the markers a decreasing degree of molecular association was observed in the BSA solutions as compared with the solutions without protein. In the solutions with BSA, fluorescence quenching and red shifting of the fluorescence spectrum maximum occurred for the solutions with BAS compared with solutions without protein for the markers of the fluorescein family. The dependences of the degree of molecular association on pH differed for fluorescein and its halogen derivatives. The efficiency dependences of nanomarker binding with BSA on pH differed for fluorescein and its halogen derivatives.
引用
收藏
页码:304 / 310
页数:7
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