Proteasome-dependent inactivation of Akt is essential for 12-O-tetradecanoylphorbol 13-acetate-induced apoptosis in vascular smooth muscle cells

被引：5

作者：

Fan, Yongna ^{[1
,2
]}

Xie, Ping ^{[1
,2
]}

Zhang, Hua ^{[1
,2
]}

Guo, Shubin ^{[3
]}

Gu, Dongfeng ^{[4
,5
,6
]}

She, Mingpeng ^{[1
,2
]}

Li, HuiHua ^{[1
,2
]}

机构：

[1] Tsinghua Univ, Chinese Acad Med Sci, Inst Basic Med Sci, Peking Union Med Coll,Dept Pathol, Beijing 100005, Peoples R China

[2] Tsinghua Univ, Chinese Acad Med Sci, Inst Basic Med Sci, Peking Union Med Coll,Natl Lab Med Mol Biol, Beijing 100005, Peoples R China

[3] Peking Union Med Hosp, Dept Acute Med, Beijing, Peoples R China

[4] Crdiovasc Inst, Div Populat Genet & Prevent, Beijing 100005, Peoples R China

[5] Chinese Acad Med Sci, Fuwai Hosp, Beijing 100005, Peoples R China

[6] Peking Union Med Coll, Beijing 100005, Peoples R China

来源：

APOPTOSIS | 2008年 / 13卷 / 12期

关键词：

Vascular smooth muscle cells; Apoptosis; 12-O-tetradecanoylphorbol; 13-acetate; Akt; Ubiquitin-proteasome system; PKC; PP2A;

D O I：

10.1007/s10495-008-0272-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apoptosis of vascular smooth muscle cells (SMCs) is a prominent feature of blood vessel remodeling. Here we investigated the effect of 12-O-tetradecanoylphorbol 13-acetate (TPA) on SMC apoptosis. We found that TPA treatment induced SMC apoptosis through the rapid downregulation of Akt phosphorylation. The inhibition of Akt activation by TPA was markedly reduced by inhibitors of protein phosphatase 2A and proteasome. Moreover, TPA promoted the ubiquitination of p-Akt, whereas inhibition of TPA-induced PKC activation suppressed the downregulation and ubiquitination of p-Akt. Taken together, these results demonstrate that TPA triggers inactivation of Akt, at least in part, through PKC and Ubiquitin-proteasome degradation, thereby contributing to SMC apoptosis.

引用

页码：1401 / 1409

页数：9

共 43 条

[31] Involvement of phorbol-12-myristate-13-acetate-induced protein 1 in goniothalamin-induced TP53-dependent and -independent apoptosis in hepatocellular carcinoma-derived cells [J].

Kuo, Kung-Kai ;

Chen, Yi-Ling ;

Chen, Lih-Ren ;

Li, Chien-Feng ;

Lan, Yu-Hsuan ;

Chang, Fang-Rong ;

Wu, Yang-Chang ;

Shiue, Yow-Ling .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2011, 256 (01) :8-23

[32] Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway [J].

Huo, Changliang ;

Wang, Li ;

Wang, Qiuli ;

Yang, Yanbo ;

Chen, Bo .

EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2021, 22 (02)

[33] Annexin V inhibits the 12-O-tetradecanoylphorbol-13-acetate-induced activation of Ras/extracellular signal-regulated kinase (ERK) signaling pathway upstream of Shc in MCF-7 cells [J].

Sato, H ;

Ogata, H ;

De Luca, LM .

ONCOGENE, 2000, 19 (25) :2904-2912

[34] Annexin V inhibits the 12-O-tetradecanoylphorbol-13-acetate-induced activation of Ras/extracellular signal-regulated kinase (ERK) signaling pathway upstream of Shc in MCF-7 cells [J].

Hirokazu Sato ;

Hideaki Ogata ;

Luigi M De Luca .

Oncogene, 2000, 19 :2904-2912

[35] Inhibition of matrix metalloproteinase-9 expression by docosahexaenoic acid mediated by heme oxygenase 1 in 12-O-tetradecanoylphorbol-13-acetate-induced MCF-7 human breast cancer cells [J].

Chen, Haw-Wen ;

Chao, Che-Yi ;

Lin, Li-Lin ;

Lu, Chia-Yang ;

Liu, Kai-Li ;

Lii, Chong-Kuei ;

Li, Chien-Chun .

ARCHIVES OF TOXICOLOGY, 2013, 87 (05) :857-869

[36] Involvement of the Akt-dependent CREB signaling pathway in hydrogen-peroxide-induced early growth response protein-1 expression in rat vascular smooth muscle cells [J].

Rondeau, Vincent ;

Jain, Ashish ;

Truong, Vanessa ;

Srivastava, Ashok K. .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2019, 97 (09) :885-892

[37] Docosahexaenoic acid-induced unfolded protein response, cell cycle arrest, and apoptosis in vascular smooth muscle cells are triggered by Ca2+-dependent induction of oxidative stress [J].

Crnkovic, Slaven ;

Riederer, Monika ;

Lechleitner, Margarete ;

Hallstroem, Seth ;

Malli, Roland ;

Graier, Wolfgang F. ;

Lindenmann, Joerg ;

Popper, Helmut ;

Olschewski, Horst ;

Olschewski, Andrea ;

Frank, Saga .

FREE RADICAL BIOLOGY AND MEDICINE, 2012, 52 (09) :1786-1795

[38] Calcitonin gene-related peptide attenuates angiotensin II-induced ROS-dependent apoptosis in vascular smooth muscle cells by inhibiting the CaMKII/CREB signalling pathway [J].

Luo, Hong-min ;

Wu, Xia ;

Liu, Wen-xuan ;

Wang, Lu-yao ;

Sun, Hong-yu ;

Zhu, Liang-yu ;

Yang, Lei .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 521 (02) :285-289

[39] Suppression of 12-O-Tetradecanoylphorbol-13-Acetate-Induced MCF-7 Breast Adenocarcinoma Cells Invasion/Migration by α-Tomatine Through Activating PKCα/ERK/NF-κB-Dependent MMP-2/MMP-9 Expressions [J].

Shi, Min-Der ;

Shih, Yuan-Wei ;

Lee, Ya-Shan ;

Cheng, Yueh-Feng ;

Tsai, Li-Yu .

CELL BIOCHEMISTRY AND BIOPHYSICS, 2013, 66 (01) :161-174

[40] Attenuation of 12-O-Tetradecanoylphorbol-13-acetate (TPA)-Induced Gap Junctional Intercellular Communication (GJIC) Inhibition in MCF-10A Cells by c9,t11-Conjugated Linoleic Acid [J].

Rakib, Md. Abdur ;

Kim, Young S. ;

Jang, Wook J. ;

Choi, Byeong D. ;

Kim, Jeong O. ;

Kong, Il K. ;

Ha, Yeong L. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (22) :12022-12030

← 1 2 3 4 5 →