Novel DNA sensor for guanine content

被引：14

作者：

Del Guerzo, A ^{[1
]}

Mesmaeker, AK ^{[1
]}

机构：

[1] Free Univ Brussels, B-1050 Brussels, Belgium

来源：

INORGANIC CHEMISTRY | 2002年 / 41卷 / 04期

关键词：

D O I：

10.1021/ic0108944

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The bifunctional complex [Ru(TAP)(2)POQ-Nmet](2+), 1, formed with a [Ru(TAP)(2)Phen](2+) metallic unit linked to a quinoline moiety, and [Ru(TAP)2Phen]2+, 2, as reference, have been tested as photoprobes of DNA. Interestingly, 1 exhibits an emission enhancement of a factor of 16-17 upon binding to calf thymus DNA. Moreover, this emission is modulated by the nucleic base content of the polynucleotide. It varies by almost an order of magnitude from a polynucleotide containing 100% of G-C to a guanine-free nucleic acid where the excited-state lifetime reaches about 2 Its. The origins of these interesting properties are analyzed by comparing 1 with reference 2 in the presence of different polynucleotides.

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页码：938 / 945

页数：8

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