Genes with increased transcript levels following harvest of the sporophore of Agaricus bisporus have multiple physiological roles

被引:28
作者
Eastwood, DC [1 ]
Kingsnorth, CS [1 ]
Jones, HE [1 ]
Burton, KS [1 ]
机构
[1] Hort Res Int, Warwick CV35 9EF, England
来源
MYCOLOGICAL RESEARCH | 2001年 / 105卷
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1016/S0953-7562(08)61993-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We screened a cDNA library generated from harvested and stored sporophores of Agaricus bisporus and identified 19 genes with higher transcript levels than at the time of harvest. Five of these genes had no detectable mRNA levels prior to detachment from the mycelium. Sequence analysis of ten clones revealed significant similarities to known genes, these code for proteins involved in polymer breakdown and metabolism, cell wall synthesis, stress tolerance, cytochrome P450 activity and DNA binding. The diversity of functions of these genes suggests the changes in the sporophore after harvest involve several different physiological processes.
引用
收藏
页码:1223 / 1230
页数:8
相关论文
共 43 条
[41]   Morphological studies on the life span, developmental stages, senescence and death of fruit bodies of Agaricus bisporus [J].
Umar, MH ;
Van Griensven, LJLD .
MYCOLOGICAL RESEARCH, 1997, 101 :1409-1422
[42]   Cytochrome P450 enzyme systems in fungi [J].
van den Brink, HJM ;
van Gorcom, RFM ;
van den Hondel, CAMJJ ;
Punt, PJ .
FUNGAL GENETICS AND BIOLOGY, 1998, 23 (01) :1-17
[43]   Expression of CEL2 and CEL4, two proteins from Agaricus bisporus with similarity to fungal cellobiohydrolase I and beta-mannanase, respectively, is regulated by the carbon source [J].
Yague, E ;
MehakZunic, M ;
Morgan, L ;
Wood, DA ;
Thurston, CF .
MICROBIOLOGY-SGM, 1997, 143 :239-244