Absolute quantification of twelve oligosaccharides in human milk using a targeted mass spectrometry-based approach

被引：42

作者：

Zhang, Wenyuan ^{[1
]}

Wang, Tong ^{[1
]}

Chen, Xinxin ^{[1
]}

Pang, Xiaoyang ^{[1
]}

Zhang, Shuwen ^{[1
]}

Ujiroghene, Obaroakpo Joy ^{[1
]}

Jiang Shilong ^{[2
]}

Jing Lu ^{[1
]}

Lv, Jiaping ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Key Lab Agrofood Proc & Qual Control, Inst Food Sci & Technol, Beijing, Peoples R China

[2] Heilongjiang Feihe Dairy Co Ltd, R&D Ctr, Beijing, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2019年 / 219卷

基金：

中国国家自然科学基金;

关键词：

Human milk oligosaccharides; Quantitative determination; Liquid chromatography - mass spectrometry; NEUTRAL OLIGOSACCHARIDE; LIQUID-CHROMATOGRAPHY; CONSUMPTION; MICROBIOTA; HEALTH; SIALYL; WOMEN;

D O I：

10.1016/j.carbpol.2019.04.092

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Human milk oligosaccharides (HMOs) are beneficial to infant's growth. Currently, the challenges were the complex operation and weak separation which limited the accurate quantification of HMOs. Liquid chromatography-mass spectrometry (LC-MS/MS) under multi-reaction monitoring (MRM) mode was developed here to separate and quantitatively determine 12 oligosaccharides in human milk. The degree of polymerization of 12 oligosaccharides ranged from 3 to 6. Among 12 HMOS, four pairs of isomeric oligosaccharides (LNFPIand LNFPIII; LSTa, LSTb and LSTc; 2FL and 3FL; 3SL and 6SL) were successfully separated. Variations of HMOs among 61 individuals from Beijing, China were observed using the present method. Pairwise correlations analysis showed high correlation in abundance between LNDFH hand 6SL, LSTc and 6SL, LNDFH II and LSTa, LSTa and LSTc, LSTa and 6SL. This method allows accurate and reliable quantitative determination of 12 oligosaccharides in human milk, and could be adopted in determining variations in HMOs among samples.

引用

页码：328 / 333

页数：6

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