A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA)

被引：318

作者：

Castoldi, M

Schmidt, S

Benes, V

Noerholm, M

Kulozik, AE

Hentze, MW

Muckenthaler, MU

机构：

[1] Heidelberg Univ, Dept Pediat Oncol Hematol & Immunol, D-69120 Heidelberg, Germany

[2] EMBL, Genom Core Facil, Heidelberg, Germany

[3] EMBL, Gene Express Unit, Heidelberg, Germany

[4] EMBL, Mol Med Partnership Unit, Heidelberg, Germany

[5] Exiqon, Res & Dev, Vedbaek, Denmark

来源：

RNA | 2006年 / 12卷 / 05期

关键词：

microRNAs; microarrays; LNA; RNA translation; mouse;

D O I：

10.1261/rna.2332406

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs represent a class of short (similar to 22 nt), noncoding regulatory RNAs involved in development, differentiation, and metabolism. We describe a novel microarray platform for genome-wide profiling of mature miRNAs (miChip) using locked nucleic acid (LNA)-modified capture probes. The biophysical properties of LNA were exploited to design probe sets for uniform, high-affinity hybridizations yielding highly accurate signals able to discriminate between single nucleotide differences and, hence, between closely related miRNA family members. The superior detection sensitivity eliminates the need for RNA size selection and/or amplification. MiChip will greatly simplify miRNA expression profiling of biological and clinical samples.

引用

收藏

页码：913 / 920

页数：8

相关论文

共 36 条

[1] The let-7 microRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans [J].

Abbott, AL ;

Alvarez-Saavedra, E ;

Miska, EA ;

Lau, NC ;

Bartel, DP ;

Horvitz, HR ;

Ambros, V .

DEVELOPMENTAL CELL, 2005, 9 (03) :403-414

[2] Probing microRNAs with microarrays: Tissue specificity and functional inference [J].

Babak, T ;

Zhang, W ;

Morris, Q ;

Blencowe, BJ ;

Hughes, TR .

RNA, 2004, 10 (11) :1813-1819

[3] MicroRNA expression detected by oligonucleotide microarrays: System establishment and expression profiling in human tissues [J].

Barad, O ;

Meiri, E ;

Avniel, A ;

Aharonov, R ;

Barzilai, A ;

Bentwich, I ;

Einav, U ;

Glad, S ;

Hurban, P ;

Karov, Y ;

Lobenhofer, EK ;

Sharon, E ;

Shiboleth, YM ;

Shtutman, M ;

Bentwich, Z ;

Einat, P .

GENOME RESEARCH, 2004, 14 (12) :2486-2494

[4] Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes [J].

Baskerville, S ;

Bartel, DP .

RNA, 2005, 11 (03) :241-247

[5] Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA [J].

Braasch, DA ;

Corey, DR .

CHEMISTRY & BIOLOGY, 2001, 8 (01) :1-7

[6] Kaposi's sarcoma-associated herpesvirus expresses an array of viral microRNAs in latently infected cells [J].

Cai, XZ ;

Lu, SH ;

Zhang, ZH ;

Gonzalez, CM ;

Damania, B ;

Cullen, BR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (15) :5570-5575

[7] Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers [J].

Calin, GA ;

Sevignani, C ;

Dan Dumitru, C ;

Hyslop, T ;

Noch, E ;

Yendamuri, S ;

Shimizu, M ;

Rattan, S ;

Bullrich, F ;

Negrini, M ;

Croce, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (09) :2999-3004

[8] MicroRNAs as regulators of mammalian hematopoiesis [J].

Chen, CZ ;

Lodish, HF .

SEMINARS IN IMMUNOLOGY, 2005, 17 (02) :155-165

[9] Mechanisms of translational control by the 3′ UTR in development and differentiation [J].

de Moor, CH ;

Meijer, H ;

Lissenden, S .

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2005, 16 (01) :49-58

[10] Specificity of microRNA target selection in translational repression [J].

Doench, JG ;

Sharp, PA .

GENES & DEVELOPMENT, 2004, 18 (05) :504-511

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