Whole Genome Sequencing Reveals Virulence Potentials ofHelicobacter pyloriStrain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma

被引：11

作者：

Mwangi, Catherine ^{[1
,2
]}

Njoroge, Stephen ^{[1
,3
]}

Tshibangu-Kabamba, Evariste ^{[4
]}

Moloo, Zahir ^{[5
]}

Rajula, Allan ^{[6
]}

Devani, Smita ^{[6
]}

Matsumoto, Takashi ^{[4
]}

Nyerere, Kimang'a ^{[1
]}

Kariuki, Samuel ^{[7
]}

Revathi, Gunturu ^{[5
]}

Yamaoka, Yoshio ^{[4
]}

机构：

[1] Jomo Kenyatta Univ Agr & Technol, Dept Med Microbiol, POB 62000-00200, Nairobi, Kenya

[2] Kenyatta Univ, Dept Med Microbiol & Parasitol, POB 43844-00100, Nairobi, Kenya

[3] Tech Univ Kenya, Dept Med Microbiol, POB 52428, Nairobi, Kenya

[4] Oita Univ, Fac Med, Dept Environm & Prevent Med, Oita 8795593, Japan

[5] Aga Khan Univ Hosp, Dept Pathol & Lab Med, POB 37002-00100, Nairobi, Kenya

[6] Aga Khan Univ Hosp, Gastroenterol Sect, POB 37002-00100, Nairobi, Kenya

[7] Kenya Govt Med Res Ctr, POB 20778-00202, Nairobi, Kenya

来源：

TOXINS | 2020年 / 12卷 / 09期

基金：

日本学术振兴会; 美国国家卫生研究院;

关键词：

Helicobacter pylori; Kenya; whole genome sequencing; virulence; virulome; mobilome; Illumina Miseq; Oxford Nanopore MinION; OUTER-MEMBRANE PROTEINS; HELICOBACTER-PYLORI; CAGA ONCOPROTEIN; DNA METHYLATION; AFRICAN ENIGMA; HOPQ ALLELES; E-CADHERIN; CANCER; INFECTION; DIFFUSE;

D O I：

10.3390/toxins12090556

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Helicobacter pylori(H.pylori) infection is etiologically associated with severe diseases including gastric cancer; but its pathogenicity is deeply shaped by the exceptional genomic diversification and geographic variation of the species. The clinical relevance of strains colonizing Africa is still debated. This study aimed to explore genomic features and virulence potentials ofH. pyloriKE21, a typical African strain isolated from a native Kenyan patient diagnosed with a gastric cancer. A high-quality circular genome assembly of 1,648,327 bp (1590 genes) obtained as a hybrid of Illumina Miseq short reads and Oxford Nanopore MinION long reads, clustered withinhpAfrica1population. This genome revealed a virulome and a mobilome encoding more than hundred features potentiating a successful colonization, persistent infection, and enhanced disease pathogenesis. Furthermore, through an experimental infection of gastric epithelial cell lines, strain KE21 showed the ability to promote interleukin-8 production and to induce cellular alterations resulting from the injection of a functional CagA oncogene protein into the cells. This study shows that strain KE21 is potentially virulent and can trigger oncogenic pathways in gastric epithelial cells. Expended genomic and clinical explorations are required to evaluate the epidemiological importance ofH. pyloriinfection and its putative complications in the study population.

引用

页数：19