Differential expression of microRNAs and potential targets under drought stress in barley

被引：71
作者：
Ferdous, Jannatul [1 ,2 ]
Sanchez-Ferrero, Juan Carlos [1 ,3 ]
Langridge, Peter [2 ]
Milne, Linda [4 ]
Chowdhury, Jamil [2 ,5 ]
Brien, Chris [3 ]
Tricker, Penny J. [1 ,2 ]
机构：
[1] Australian Ctr Plant Funct Genom, PMB1, Glen Osmond, SA 5064, Australia
[2] Univ Adelaide, Sch Agr Food & Wine, PMB1, Glen Osmond, SA 5064, Australia
[3] Univ South Australia, Sch Informat Technol & Math Sci, Phen & Bioinformat Res Ctr, Mawson Lakes, SA 5095, Australia
[4] James Hutton Inst, Dundee DD2 5DA, Scotland
[5] ARC Ctr Excellence Plant Cell Walls, PMB1, Glen Osmond, SA 5064, Australia
来源：
PLANT CELL AND ENVIRONMENT | 2017年 / 40卷 / 01期
关键词：
Hordeum vulgare; canonical cleavage; degradome; genotype; in situ-RT-PCR; SMALL RNAS; RT-PCR; RESPONSIVE MICRORNAS; TRANSCRIPTION FACTOR; GENE-EXPRESSION; MESSENGER-RNA; IDENTIFICATION; ARABIDOPSIS; MIRNAS; WATER;
D O I：
10.1111/pce.12764
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Drought is a crucial environmental constraint limiting crop production in many parts of the world. microRNA (miRNA) based gene regulation has been shown to act in several pathways, including crop response to drought stress. Sequence based profiling and computational analysis have revealed hundreds of miRNAs and their potential targets in different plant species under various stress conditions, but few have been biologically verified. In this study, 11 candidate miRNAs were tested for their expression profiles in barley. Differences in accumulation of only four miRNAs (Ath-miR169b, Osa-miR1432, Hv-miRx5 and Hv-miR166b/c) were observed between drought-treated and well-watered barley in four genotypes. miRNA targets were predicted using degradome analysis of two, different genotypes, and genotype-specific target cleavage was observed. Inverse correlation of mature miRNA accumulation with miRNA target transcripts was also genotype dependent under drought treatment. Drought-responsive miRNAs accumulated predominantly in mesophyll tissues. Our results demonstrate genotype-specific miRNA regulation under drought stress and evidence for their role in mediating expression of target genes for abiotic stress response in barley.
引用
收藏
页码：11 / 24
页数：14
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