Dyella humi sp nov., isolated from forest soil

A Gram-stain-negative, aerobic, motile, rod-shaped, yellow-pigmented bacterium, designated as DHG40(T), was isolated from a soil sample collected from the forest of Dinghushan Biosphere Reserve, Guangdong Province, China. Strain DHG40(T) grew at pH 4.0-8.0 and 10-37 degrees C (optimum at pH 6.0-7.0 and 25-28 degrees C). NaCl inhibited growth at concentrations above 2.5% (w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate fell within the cluster of the genus Dyella. Strain DHG40(T) was closely related to Dyella ginsengisoli Gsoil 3046(T) (97.6% 16S rRNA gene sequence similarity), Dyella marensis CS5-B2(T) (97.5 %), Dyella koreensis BB4(T) (97.4 %) and Dyella jejuensis JP1(T) (97.4 %). The DNA-DNA relatedness values between strain DHG40(T) and its phylogenetically closest relatives were all below 40 %. The DNA G+C content was 60.3 mol%. In addition, iso-C-15 : 0, iso-C-16 : 0, iso-C-17 : 0 and iso-C-17 (: 1)omega 9c were the major fatty acids (> 10 %) and ubiquinone-8 was the respiratory quinone. The major polar lipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminolipid and an unidentified phospholipid. On the basis of phylogenetic, phenotypic, physiological and chemotaxonomic distinctiveness, strain DHG40T should be placed in the genus Dyella as a representative of a novel species, for which the name Dyella humi sp. nov. is proposed. The type strain is DHG40(T) (=KCTC 42629(T) =LMG 28842(T)).