Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dur)

被引:38
作者
An, Xing-Kui [1 ]
Sun, Liang [1 ,2 ]
Liu, Hang-Wei [1 ]
Liu, Dan-Feng [1 ]
Ding, Yu-Xiao [1 ,2 ,3 ]
Li, Le-Mei [1 ]
Zhang, Yong-Jun [1 ]
Guo, Yu-Yuan [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
[2] Chinese Acad Agr Sci, Tea Res Inst, Key Lab Tea Biol & Resources Utilizat, Minist Agr, Hangzhou 310008, Zhejiang, Peoples R China
[3] Jilin Agr Univ, Coll Agron, Changchun 130118, Jilin, Peoples R China
来源
SCIENTIFIC REPORTS | 2016年 / 6卷
基金
中国国家自然科学基金;
关键词
CANDIDATE PHEROMONE RECEPTORS; DROSOPHILA ODORANT RECEPTORS; FUNCTIONAL-CHARACTERIZATION; SOLUBLE-PROTEINS; BT COTTON; MIRID BUG; HEMIPTERA; EVOLUTION; SYSTEM; ORCO;
D O I
10.1038/srep37870
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Olfactory receptors are believed to play a central role in insects host-seeking, mating, and ovipositing. On the basis of male and female antennal transcriptome of adult Apolygus lucorum, a total of 110 candidate A. lucorum odorant receptors (AlucOR) were identified in this study including five previously annotated AlucORs. All the sequences were validated by cloning and sequencing. Tissue expression profiles analysis by RT-PCR indicated most AlucORs were antennal highly expressed genes. The qPCR measurements further revealed 40 AlucORs were significantly higher in the antennae. One AlucOR was primarily expressed in the female antennae, while nine AlucORs exhibited male-biased expression patterns. Additionally, both the RPKM value and RT-qPCR analysis showed AlucOR83 and AlucOR21 were much higher abundant in male antennae than in female antennae, suggesting their different roles in chemoreception of gender. Phylogenetic analysis of ORs from several Hemipteran species demonstrated that most AlucORs had orthologous genes, and five AlucOR-specific clades were defined. In addition, a sub-clade of potential male- based sex pheromone receptors were also identified in the phylogenetic tree of AlucORs. Our results will facilitate the functional studies of AlucORs, and thereby provide a foundation for novel pest management approaches based on these genes.
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页数:12
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