Clostridium carboxidivorans Strain P7T Recombinant Formate Dehydrogenase Catalyzes Reduction of CO2 to Formate

被引:63
作者
Alissandratos, Apostolos [1 ]
Kim, Hye-Kyung [1 ]
Matthews, Hayden [1 ]
Hennessy, James E. [1 ]
Philbrook, Amy [1 ]
Easton, Christopher J. [1 ]
机构
[1] Australian Natl Univ, Res Sch Chem, CSIRO Biofuels Res Cluster, Canberra, ACT, Australia
关键词
MOLYBDENUM; ACETATE;
D O I
10.1128/AEM.02886-12
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Recombinant formate dehydrogenase from the acetogen Clostridium carboxidivorans strain P7(T), expressed in Escherichia coli, shows particular activity towards NADH-dependent carbon dioxide reduction to formate due to the relative binding affinities of the substrates and products. The enzyme retains activity over 2 days at 4 degrees C under oxic conditions.
引用
收藏
页码:741 / 744
页数:4
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