Total phenolics, flavonoids and antioxidant potential of organic extract of fresh water algal sample collected from a marine lake

In the present study, we carried out a research on fresh water algal sample of upper-lake of Bhopal, Madhya Pradesh, India. Several different species of fresh water mixture algal samples were collected such as cyrophyceae (46.17%), chlorophyceae (39.27%) and bavillarior phyceae (26.65%). Further, the collected algal material was extracted with 70% methanol and analyzed for its total phenolic content and phytochemical properties. Furthermore, the methanolic extract was evaluated for its in vitro antioxidant activity by using different assays such as DPPH radical scavenging activity, hydrogen peroxide scavenging activity and reducing power assay. Measurement of total phenolic content of the methanolic extract of mixed algal sample was achieved by using Folin-Ciocalteau reagent containing 134 +/- 0.91 mg GAE g(-1) of phenolic content, which was found significantly potent when compared to reference standard gallic acid. The flavonoid content of methanolic extract of mixed algal sample was found to be 20.11 +/- 2.13 mg QE g(-1). The DPPH activity of methanolic extract of mixed algal sample (20-100 mu g mL(-1)) was increased in a dose-dependent manner, which was found in the range of 45.68-67.68% as compared to ascorbic acid 64.26-82.58%. The results obtained in this study clearly indicate that fresh water algal sample has a significant potential to be used as a supplement of antioxidant agent.