Quantitative determination of paclitaxel and its metabolites, 6α-hydroxypaclitaxel and p-3′-hydroxypaclitaxel, in human plasma using column-switching liquid chromatography/tandem mass spectrometry

被引：13

作者：

Yamaguchi, Hiroaki ^{[1
]}

Fujikawa, Asuka ^{[1
]}

Ito, Hajime ^{[1
]}

Tanaka, Nobuaki ^{[1
]}

Furugen, Ayako ^{[1
]}

Miyamori, Kazuaki ^{[1
]}

Takahashi, Natsuko ^{[2
]}

Ogura, Jiro ^{[1
]}

Kobayashi, Masaki ^{[1
]}

Yamada, Takehiro ^{[3
]}

Mano, Nariyasu ^{[4
]}

Iseki, Ken ^{[1
,3
]}

机构：

[1] Hokkaido Univ, Fac Pharmaceut Sci, Sapporo, Hokkaido 0600812, Japan

[2] Hokkaido Univ, Grad Sch Med, Sapporo, Hokkaido 0608638, Japan

[3] Hokkaido Univ Hosp, Dept Pharm, Sapporo, Hokkaido 0608648, Japan

[4] Tohoku Univ Hosp, Dept Pharmaceut Sci, Sendai, Miyagi 9808574, Japan

来源：

BIOMEDICAL CHROMATOGRAPHY | 2013年 / 27卷 / 04期

关键词：

paclitaxel; metabolite; human plasma; column-switching; QUANTIFICATION; ASSAY;

D O I：

10.1002/bmc.2826

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A column-switching liquid chromatography/electrospray ionization tandem mass spectrometry to determine paclitaxel and its metabolites, 6-hydroxypaclitaxel and p-3-hydroxypaclitaxel, in human plasma was developed. The analytical system had a Shim-Pack MAYI-ODS (10x4.6mm i.d.) trapping column with deproteinization ability that concentrates analytes and removes water-soluble components. This method covered a linearity range of 55000ng/mL of concentrations in plasma for paclitaxel, a range of 0.87870ng/mL for 6-hydroxypaclitaxel and a range of 0.87435ng/mL for p-3-hydroxypaclitaxel. The intra-day precision and inter-day precision of analysis were less than 11.1%, and the accuracy was within +/- 14.4% at concentrations of 5, 50, 500 and 5000ng/mL for paclitaxel, 0.87, 8.7, 87 and 870ng/mL for 6-hydroxypaclitaxel, and 0.87, 8.7, 87 and 435ng/mL for p-3-hydroxypaclitaxel. The total run time was 30min. Our method was successfully applied to clinical pharmacokinetic investigation. Copyright (c) 2012 John Wiley & Sons, Ltd.

引用

页码：539 / 544

页数：6

共 15 条

[1] Determination of the vaporization of solutions of mutagenic antineoplastic agents at 23 and 37°C, using a desiccator technique [J].