Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers

被引:7
|
作者
Reid, Paul [1 ,2 ]
Staudacher, Alexander H. [3 ,4 ,5 ]
Marcu, Loredana G. [1 ,6 ]
Olver, Ian [5 ]
Moghaddasi, Leyla [7 ,8 ]
Brown, Michael P. [3 ,4 ,9 ,10 ]
Li, Yanrui [2 ]
Bezak, Eva [1 ,2 ,7 ]
机构
[1] Univ South Australia, Sch Hlth Sci, Adelaide, SA 5001, Australia
[2] Univ South Australia, Canc Res Inst, Adelaide, SA 5001, Australia
[3] SA Pathol, Ctr Canc Biol, Translat Oncol Lab, Adelaide, SA 5000, Australia
[4] Univ South Australia, Adelaide, SA 5000, Australia
[5] Univ Adelaide, Sch Psychol, Adelaide, SA 5000, Australia
[6] Univ Oradea, Fac Sci, Oradea 410087, Romania
[7] Univ Adelaide, Dept Phys, Adelaide, SA 5005, Australia
[8] Genesis Care, Adelaide Radiotherapy Ctr, Adelaide, SA 5000, Australia
[9] Univ Adelaide, Sch Med, Adelaide, SA 5000, Australia
[10] Royal Adelaide Hosp, Canc Clin Trials Unit, Adelaide, SA 5000, Australia
关键词
head and neck cancer; HNSCCC; HPV; radiosensitivity; clonogenic assay; fractionated radiation therapy; SQUAMOUS-CELL CARCINOMA; HUMAN-PAPILLOMAVIRUS; RADIOTHERAPY; RADIATION; BIOLOGY; REPAIR; LINES;
D O I
10.3390/cells9081788
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Head and neck squamous cell carcinomas (HNSCC) resulting from human papillomavirus (HPV) are increasing in incidence but demonstrate significantly better treatment response than HNSCC from other causes such as tobacco and alcohol. This study sought to identify differences in HNSCC, intrinsic to HPV status, in their response to radiation dose. Previously unexamined changes in radio-responsiveness following fractionated X-ray irradiation were compared between HPV positive and negative statuses of HNSCC. Six HNSCC cell lines, 3 of each HPV status, were investigated for radiosensitivity by clonogenic assay and modelled by response as a function of dose. Generational cultures of each cell line were developed to follow changes in radiosensitivity after repeated irradiations simulating fractionated radiation therapy. As a group, the HPV positive cell lines were more radiosensitive, but with changes following repeated fractions of dose, and modelling of response as a function of dose, both statuses displayed large radiobiological heterogeneity. These findings challenge current radiobiological assumptions of head and neck cancers as early responding tissue to radiation and may go some way in explaining difficulties reaching consensus in stratification of treatment by HPV status. Consequently, results from this study do not support stratifying radiation therapy by HPV status.
引用
收藏
页数:14
相关论文
共 50 条
  • [21] Characteristic differences in radiation-induced DNA damage response in human papillomavirus-negative and human papillomavirus-positive head and neck cancers with accumulation of fractional radiation dose
    Reid, Paul
    Staudacher, Alexander H.
    Marcu, Loredana G.
    Olver, Ian
    Moghaddasi, Leyla
    Brown, Michael P.
    Bezak, Eva
    HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 2021, 43 (10): : 3086 - 3096
  • [22] Prognosis of HPV-positive head and neck cancers: implication of smoking and immunosuppression
    Duray, Anaelle
    Lacremans, Daniel
    Demoulin, Stephanie
    Delvenne, Philippe
    Saussez, Sven
    ADVANCES IN CELLULAR AND MOLECULAR OTOLARYNGOLOGY, 2014, 2 (01)
  • [23] Oncolytic activity of reovirus in HPV positive and negative head and neck squamous cell carcinoma
    Timothy Cooper
    Vincent L Biron
    David Fast
    Raymond Tam
    Thomas Carey
    Maya Shmulevitz
    Hadi Seikaly
    Journal of Otolaryngology - Head & Neck Surgery, 44
  • [24] Development and Characterization of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinoma Tumorgrafts
    Kimple, Randall J.
    Harari, Paul M.
    Torres, Alexandra D.
    Yang, Robert Z.
    Soriano, Benjamin J.
    Yu, Menggang
    Armstrong, Eric A.
    Blitzer, Grace C.
    Smith, Molly A.
    Lorenz, Laurel D.
    Lee, Denis
    Yang, David T.
    McCulloch, Timothy M.
    Hartig, Gregory K.
    Lambert, Paul F.
    CLINICAL CANCER RESEARCH, 2013, 19 (04) : 855 - 864
  • [25] Salivary miRNA panel to detect HPV-positive and HPV-negative head and neck cancer patients
    Wan, Yunxia
    Vagenas, Dimitrios
    Salazar, Carolina
    Kenny, Liz
    Perry, Chris
    Calvopina, Diego
    Punyadeera, Chamindie
    ONCOTARGET, 2017, 8 (59) : 99990 - 100001
  • [26] Treatment outcomes in veterans with HPV-positive head and neck cancer
    Feinstein, Aaron J.
    Shay, Sophie G.
    Chang, Elena
    Lewis, Michael S.
    Wang, Marilene B.
    AMERICAN JOURNAL OF OTOLARYNGOLOGY, 2017, 38 (02) : 188 - 192
  • [27] New treatment strategies for HPV-positive head and neck cancer
    Kofler, B.
    Laban, S.
    Busch, C. J.
    Loerincz, B.
    Knecht, R.
    EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, 2014, 271 (07) : 1861 - 1867
  • [28] Difference Between HPV-Positive and HPV-Negative Non-Oropharyngeal Head and Neck Cancer: Texture Analysis Features on CT
    Fujita, Akifumi
    Buch, Karen
    Li, Baojun
    Kawashima, Yusuke
    Qureshi, Muhammad M.
    Sakai, Osamu
    JOURNAL OF COMPUTER ASSISTED TOMOGRAPHY, 2016, 40 (01) : 43 - 47
  • [29] The Key Differences between Human Papillomavirus-Positive and -Negative Head and Neck Cancers: Biological and Clinical Implications
    Powell, Steven F.
    Vu, Lexi
    Spanos, William C.
    Pyeon, Dohun
    CANCERS, 2021, 13 (20)
  • [30] The Antiviral Agent Cidofovir Induces DNA Damage and Mitotic Catastrophe in HPV-Positive and -Negative Head and Neck Squamous Cell Carcinomas In Vitro
    Verhees, Femke
    Legemaate, Dion
    Demers, Imke
    Jacobs, Robin
    Haakma, Wisse Evert
    Rousch, Mat
    Kremer, Bernd
    Speel, Ernst Jan
    CANCERS, 2019, 11 (07)