Reduced CD4 T Lymphocytes in Lymph Nodes of the Mouse Model of Autism Induced by Valproic Acid

被引:4
|
作者
Baronio, Diego [1 ,2 ,7 ]
Bauer-Negrini, Guilherme [1 ,3 ,4 ]
Castro, Kamila [1 ,2 ]
Nunes, Gustavo Della-Flora [1 ,3 ]
Riesgo, Rudimar [1 ,2 ,4 ]
Mendes-da-Cruz, Daniella Areas [4 ,5 ]
Savino, Wilson [4 ,5 ]
Gottfried, Carmem [1 ,3 ,4 ]
Bambini-Junior, Victorio [1 ,4 ,6 ]
机构
[1] Clin Hosp Porto Alegre, Translat Grp Autism Spectrum Disorder GETTEA, Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Clin Hosp Porto Alegre, Child Neurol Unit, Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Dept Biochem, Porto Alegre, RS, Brazil
[4] Brazilian Natl Inst Sci & Technol Neuroimmunomodu, Rio De Janeiro, Brazil
[5] Fundacao Oswaldo Cruz, Oswaldo Cruz Inst, Lab Thymus Res, Rio De Janeiro, Brazil
[6] Univ Cent Lancashire, Sch Pharm & Biomed Sci, Maudland Bldg,MB241, Preston PR1 2XT, Lancs, England
[7] Univ Helsinki, Dept Anat, Haartmaninkatu 8, FI-00014 Helsinki, Finland
关键词
Autism spectrum disorder; Valproic acid; Lymph nodes; SPECTRUM DISORDERS; ANIMAL-MODEL; CHILDREN; RISK; CHILDHOOD; RESPONSES; BEHAVIOR; EXPOSURE; MEMORY; CELLS;
D O I
10.1159/000491395
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: Considering the potential role of lymphocytes in the pathophysiology of autism spectrum disorder (ASD), we aimed to evaluate possible alterations of T cell pools in the lymphoid organs of an animal model of autism induced by valproic acid (VPA). Pregnant Swiss mice received a single intraperitoneal injection of 600 mg/kg of VPA (VPA group) or saline (control group) on day 11 of gestation. Male offspring were euthanized on postnatal day 60 for removal of thymuses, spleens, and a pool of inguinal, axillary and brachial lymph nodes. Cellularity was evaluated, and flow cytometry analysis was performed on cell suspensions incubated with the mouse antibodies anti-CD3-FITC, anti-CD4-PE, and anti-CD8-PE-Cy7. We observed that the prenatal exposure to VPA induced a reduction in the numbers of CD3+CD4+ T cells in their lymph nodes when compared to the control animals. This was specific since it was not seen in the thymus or spleen. The consistent decrease in the number of CD4+ T cells in subcutaneous lymph nodes of mice from the animal model of autism may be related to the allergic symptoms frequently observed in ASD. Further research is necessary to characterize the immunological patterns in ASD and the connection with the pathophysiology of this disorder. (C) 2018 S. Karger AG, Basel
引用
收藏
页码:280 / 284
页数:5
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